High-fat diet-induced adiposity, adipose inflammation, hepatic steatosis and hyperinsulinemia in outbred CD-1 mice

PloS One
M GaoD Liu

Abstract

High-fat diet (HFD) has been applied to a variety of inbred mouse strains to induce obesity and obesity related metabolic complications. In this study, we determined HFD induced development of metabolic disorders on outbred female CD-1 mice in a time dependent manner. Compared to mice on regular chow, HFD-fed CD-1 mice gradually gained more fat mass and consequently exhibited accelerated body weight gain, which was associated with adipocyte hypertrophy and up-regulated expression of adipose inflammatory chemokines and cytokines such as Mcp-1 and Tnf-α. Increased fat accumulation in white adipose tissue subsequently led to ectopic fat deposition in brown adipose tissue, giving rise to whitening of brown adipose tissue without altering plasma level of triglyceride. Ectopic fat deposition was also observed in the liver, which was associated with elevated expression of key genes involved in hepatic lipid sequestration, including Ppar-γ2, Cd36 and Mgat1. Notably, adipose chronic inflammation and ectopic lipid deposition in the liver and brown fat were accompanied by glucose intolerance and insulin resistance, which was correlated with hyperinsulinemia and pancreatic islet hypertrophy. Collectively, these results demonstrate sequenti...Continue Reading

References

Sep 1, 1988·Diabetes·R S SurwitM N Feinglos
Nov 15, 2001·Obesity Research·P T JamesM Shayeghi
Jun 7, 2002·Australasian Physical & Engineering Sciences in Medicine·H ChenJ L Hu
Dec 12, 2003·The British Journal of Nutrition·Susanna IossaGiovanna Liverini
Dec 18, 2003·The Journal of Clinical Investigation·Stuart P WeisbergAnthony W Ferrante
Dec 18, 2003·The Journal of Clinical Investigation·Haiyan XuHong Chen
Oct 2, 2004·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·J S Mayes, G H Watson
Apr 13, 2007·The New England Journal of Medicine·Claus M LarsenMarc Y Donath
Jul 20, 2007·The New England Journal of Medicine·Saul Malozowski, Jon Todd Sahlroot
Apr 18, 2008·Gene Therapy·T-C Liu, D Kirn
Mar 28, 2009·PLoS Computational Biology·Junghyo JoVipul Periwal
Apr 10, 2009·The New England Journal of Medicine·Wouter D van Marken LichtenbeltG J Jaap Teule
Apr 10, 2009·The New England Journal of Medicine·Aaron M CypessC Ronald Kahn
Apr 10, 2009·The New England Journal of Medicine·Kirsi A VirtanenPirjo Nuutila
Feb 13, 2010·Annual Review of Physiology·Jerrold M Olefsky, Christopher K Glass
Apr 30, 2010·Laboratory Animals·W L BreslinBrian K McFarlin
Jan 25, 2011·Nature Medicine·Alexander BarteltJoerg Heeren
Jun 3, 2011·The Journal of Clinical Investigation·Kai SunPhilipp E Scherer
Jun 3, 2011·The Journal of Clinical Investigation·Carey N Lumeng, Alan R Saltiel
Sep 14, 2011·Biochemical and Biophysical Research Communications·Neha GargMartin L Adamo
Jan 13, 2012·The Journal of Clinical Endocrinology and Metabolism·Stephan J Guyenet, Michael W Schwartz
Feb 4, 2012·Genes & Development·Ffolliott M FisherBruce M Spiegelman
Feb 9, 2012·Diabetes·Subhadra C Gunawardana, David W Piston
Mar 6, 2012·Cell·Varman T Samuel, Gerald I Shulman
Jul 17, 2012·Advances in Nutrition·Rebecca Kanter, Benjamin Caballero
Nov 21, 2012·Nature Reviews. Endocrinology·Vasanti S MalikFrank B Hu
Dec 12, 2012·Cell Metabolism·Arya E MehranJames D Johnson
Dec 12, 2012·The Journal of Clinical Investigation·Kristin I StanfordLaurie J Goodyear
Jan 11, 2013·Physiological Reviews·André Tchernof, Jean-Pierre Després
Apr 18, 2013·The AAPS Journal·Mingming Gao, Dexi Liu
May 29, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Christine PodriniJacqueline K White
Jun 6, 2013·American Journal of Physiology. Endocrinology and Metabolism·Michael S F WiedemannDaniel Konrad
Jun 19, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mingming GaoDexi Liu
Aug 2, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lori Asarian, Nori Geary
Aug 8, 2013·Journal of Cardiovascular Pharmacology·Armen YerevanianRoger J Hajjar

❮ Previous
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Citations

Jun 5, 2016·Asian Pacific Journal of Tropical Medicine·Hyeon-Jeong KimMyung-Haing Cho
Jan 30, 2016·Diabetes·Alice MarmugiIsabelle Leclerc
Apr 27, 2016·International Journal of Obesity : Journal of the International Association for the Study of Obesity·T WangL Qi
Mar 10, 2016·The Journal of Endocrinology·Renato Simões GasparAntonio Marcus de Andrade Paes
Feb 22, 2017·Basic & Clinical Pharmacology & Toxicology·Vivi F H JensenIngrid B Bøgh
May 19, 2017·The American Journal of Chinese Medicine·Hyeon-Jeong KimMyung-Haing Cho
Apr 16, 2017·Journal of Lipid Research·Stephen M StoreyFriedhelm Schroeder
Jan 20, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zachary I GrunewaldJaume Padilla
Jul 26, 2018·Food & Function·Xue HanWeidong Huang
Jul 25, 2017·Molecular Nutrition & Food Research·Hui ChengQinglin Li
Nov 25, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nathan C WinnJaume Padilla
Nov 1, 2015·Clinical Science·Hyo Joon KimBert Binas
Oct 13, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jean-Francois LandrierRalph Rühl
Nov 21, 2019·Journal of Medicinal Food·Angélica S González-GaribayBlanca E Bastidas-Ramírez
Nov 29, 2016·Oncotarget·Nicola Di DanieleAntonino De Lorenzo
Apr 13, 2018·International Journal of Molecular Sciences·Jingyi CaoHaifei Shi
Sep 27, 2018·Journal of Neuroinflammation·Estella A NewcombeRodrigo Medeiros
Feb 15, 2019·Frontiers in Endocrinology·Anup BhusalKyoungho Suk
Oct 27, 2017·American Journal of Physiology. Endocrinology and Metabolism·Ivan Torre-VillalvazoGerardo Gamba
Nov 3, 2017·Physiological Genomics·Y ChenT Casey
Sep 11, 2020·BioMed Research International·Jin CaiFei Huang
Aug 9, 2016·International Journal of Molecular Medicine·Youngmin KimTaek-Jeong Nam
Jul 3, 2019·Journal of Cellular Physiology·Tsan-Ju ChenHui-Shan Hung

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Methods Mentioned

BETA
ELISA
electrophoresis
PCR

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