High-Fat Diet-Induced Retinal Dysfunction

Investigative Ophthalmology & Visual Science
Richard Cheng-An ChangGladys Y-P Ko

Abstract

The purpose of this study was to investigate the impact of obesity-induced prediabetes/early diabetes on the retina to provide new evidence on the pathogenesis of type 2 diabetes-associated diabetic retinopathy (DR). A high-fat diet (HFD)-induced obesity mouse model (male C57BL/6J) was used in this study. At the end of the 12-week HFD feeding regimen, mice were evaluated for glucose and insulin tolerance, and retinal light responses were recorded by electroretinogram (ERG). Western immunoblot and immunohistochemical staining were used to determine changes in elements regulating calcium homeostasis between HFD and control retinas, as well as unstained human retinal sections from DR patients and age-appropriate controls. Compared to the control, the scotopic and photopic ERGs from HFD mice were decreased. There were significant decreases in molecules related to cell signaling, calcium homeostasis, and glucose metabolism from HFD retinas, including phosphorylated protein kinase B (pAKT), glucose transporter 4, L-type voltage-gated calcium channel (L-VGCC), and plasma membrane calcium ATPase (PMCA). Similar changes for pAKT, PMCA, and L-VGCC were also observed in human retinal sections from DR patients. Obesity-induced hyperglycemi...Continue Reading

Citations

Apr 3, 2016·General and Comparative Endocrinology·Thomas FlemingSteve Harvey
Jun 2, 2017·Physiological Reviews·Nicholas StaffordElizabeth J Cartwright
Dec 21, 2017·Frontiers in Molecular Neuroscience·Liheng ShiGladys Y-P Ko
Jun 22, 2018·Disease Models & Mechanisms·Meng ZhangNady Golestaneh
Jun 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiwei DaiMichael D Dennis
Feb 6, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Bjorn T TamSylvia Santosa
Jul 22, 2018·Current Eye Research·Basma BaccoucheRafika Ben Chaouacha-Chekir
Dec 31, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yuxiang DuHongsheng Bi
May 18, 2019·Journal of Diabetes Research·Janet Ya-An ChangGladys Y-P Ko
Nov 9, 2019·Journal of the American Heart Association·Liheng ShiGladys Y-P Ko
Jan 26, 2020·Journal of Clinical Medicine·Reem T AtawiaR William Caldwell
Jan 15, 2021·Experimental Eye Research·Danielle A Clarkson-TownsendMachelle T Pardue
Jan 29, 2021·International Journal of Molecular Sciences·Yuta SakamotoSatoshi Shimo
May 11, 2020·Experimental Eye Research·Mayssa AlboueryMarie-Agnès Bringer

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