High fat feeding affects the number of GPR120 cells and enteroendocrine cells in the mouse stomach

Frontiers in Physiology
Patricia WidmayerHeinz Breer

Abstract

Long-term intake of dietary fat is supposed to be associated with adaptive reactions of the organism and it is assumptive that this is particularly true for fat responsive epithelial cells in the mucosa of the gastrointestinal tract. Recent studies suggest that epithelial cells expressing the receptor for medium and long chain fatty acids, GPR120 (FFAR4), may operate as fat sensors. Changes in expression level and/or cell density are supposed to be accompanied with a consumption of high fat (HF) diet. To assess whether feeding a HF diet might impact on the expression of fatty acid receptors or the number of lipid sensing cells as well as enteroendocrine cell populations, gastric tissue samples of non-obese and obese mice were compared using a real time PCR and immunohistochemical approach. In this study, we have identified GPR120 cells in the corpus region of the mouse stomach which appeared to be brush cells. Monitoring the effect of HF diet on the expression of GPR120 revealed that after 3 weeks and 6 months the level of mRNA for GPR120 in the tissue was significantly increased which coincided with and probably reflected a significant increase in the number of GPR120 positive cells in the corpus region; in contrast, within th...Continue Reading

References

Nov 1, 1983·Biochimica Et Biophysica Acta·T H LiaoM Hamosh
Feb 14, 1995·Regulatory Peptides·M Furuse, G J Dockray
Jan 25, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M Covasa, R C Ritter
Apr 6, 2001·Diabetes·M TschöpM L Heiman
Apr 12, 2002·Biochemical and Biophysical Research Communications·Bernard BeckAlain Stricker-Krongrad
Nov 1, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·K A BoydC Feinle
May 8, 2003·Anatomia, histologia, embryologia·S K KuJ H Lee
Jul 17, 2003·The Keio Journal of Medicine·Irvin M ModlinN A Wright
Oct 12, 2004·Clinics in Dermatology·George A Bray
Dec 28, 2004·Nature Medicine·Akira HirasawaGozoh Tsujimoto
Mar 19, 2005·The Journal of Biological Chemistry·Susumu KatsumaGozoh Tsujimoto
Aug 14, 2008·The Journal of Clinical Endocrinology and Metabolism·Thomas FischerStefan Schulz
Dec 17, 2008·Neuroscience Letters·Shigenobu MatsumuraTohru Fushiki
Mar 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wahiba NeftiNicolas Darcel
Apr 30, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·N P HylandK A Sharkey
Jun 25, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cristina CartoniSami Damak
Apr 26, 2011·Physiology & Behavior·Diana L WilliamsAnn-Marie Torregrossa
May 12, 2011·European Journal of Endocrinology·Fabiana A N MaksudAlfredo J A Barbosa
Jun 21, 2011·Histochemistry and Cell Biology·Milena Saqui-SalcesJuanita L Merchant
Nov 16, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·P WidmayerH Breer
Apr 21, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Milena Saqui-SalcesJuanita L Merchant
May 2, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F A DucaM Covasa
Jun 9, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xinping LuStephen Wank
Sep 18, 2013·The Journal of Nutritional Biochemistry·Frank A DucaMihai Covasa
Nov 14, 2013·American Journal of Physiology. Endocrinology and Metabolism·Zhi GongIchiro Sakata
Dec 12, 2013·Molecular Metabolism·Maja S EngelstoftThue W Schwartz
May 6, 2014·Frontiers in Physiology·Julia Anna-Maria EberleHeinz Breer

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Citations

Oct 7, 2015·Frontiers in Physiology·Amelie T RettenbergerDésireé Haid
Mar 3, 2020·Frontiers in Physiology·Patricia WidmayerHeinz Breer
Jun 23, 2020·International Journal of Endocrinology·Yonas AkaluBirhanu Ayelign
Dec 31, 2020·International Journal of Molecular Sciences·Patrick O'BrienChristopher P Corpe

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Methods Mentioned

BETA
dissection
PCR

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LightCycler
GraphPad Prism
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