High-fidelity of non-small cell lung cancer xenograft models derived from bronchoscopy-guided biopsies

Thoracic Cancer
Shuai FuJie Wang

Abstract

At present, there are two main types of lung cancer xenograft models: those derived from stable cell lines, and patient-derived xenograft models established by surgically resected tissues. However, these animal models may not reflect the biological and genetic characteristics of advanced non-small cell lung cancer (NSCLC). We utilized bronchoscopy-guided biopsy tissues of NSCLC patients to establish xenograft models and analyzed their histopathologic and genotypic fidelity with parental tumors. Tumor tissues of NSCLC patients taken via bronchoscope were subcutaneously implanted into mice with non-obese diabetic-severe combined immunodeficiency disease for model establishment and serial passage. The histopathology and genotype of the samples from bronchoscopy-guided biopsy-derived xenograft (BDX) models and their parental tumors were detected. Thirty BDXs out of 114 NSCLC patients (26.32%) were successfully established. Smoking status significantly affected the success rate of NSCLC BDX establishment (P = 0.010). The BDX establishment success rate in squamous cell cancer was higher than in adenocarcinoma, with no significant difference (32.00% vs. 16.21%, P = 0.112). However, the growth rate of passage 1 BDX was slower than that...Continue Reading

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Citations

Mar 3, 2018·Journal of Clinical Medicine·Juliet A Williams
Feb 3, 2018·Expert Opinion on Drug Discovery·Aditi P SinghHaiying Cheng
Feb 14, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jiangyong YuJie Wang

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Methods Mentioned

BETA
genotyping
xenograft
biopsies
biopsy
xenografts

Software Mentioned

Graphpad Prism

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