High-field NMR studies of molecular recognition and structure-function relationships in antimicrobial piscidins at the water-lipid bilayer interface

Journal of the American Chemical Society
Eduard Y ChekmenevMyriam Cotten

Abstract

High magnetic field solid-state NMR was performed on amphipathic cationic antimicrobial peptides from fish to characterize their secondary structure and orientation in hydrated phospholipid bilayers. High-resolution distance and orientational restraints on 13C- and 15N-labeled amidated piscidins 1 and 3 provided site-specific information establishing alpha-helicity and an orientation parallel to the membrane surface. Few membrane-bound natural peptides with this topology have been structurally studied at high resolution in the presence of hydrated lipid bilayers. This orientation was foreseen since the partitioning of amphipathic cationic antimicrobial peptides at the water-bilayer interface allows for favorable peptide-lipid interactions, and it may be related to the mechanism of action. The enhanced resolution obtained at 900 MHz evidences a determinant advantage of ultra-high-field NMR for the structural determination of multiple-labeled peptides and proteins.

References

Mar 13, 1999·Biophysical Chemistry·P C Biggin, M S Sansom
Nov 20, 2001·Nature·U Silphaduang, E J Noga
Jan 25, 2002·Nature·Michael Zasloff
Jan 30, 2002·FEMS Microbiology Letters·Robert E W Hancock, Annett Rozek
Jul 19, 2002·Biophysical Journal·Kevin J HallockA Ramamoorthy
Dec 20, 2002·Biopolymers·Yechiel Shai
May 28, 2003·Biochemistry·Katherine A Henzler WildmanA Ramamoorthy
Dec 9, 2003·IUBMB Life·Alison Drechsler, Frances Separovic
Mar 17, 2004·Peptides·Jon-Paul S Powers, Robert E W Hancock
Aug 12, 2004·Chemical Reviews·Stanley J Opella, Francesca M Marassi
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden

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Citations

Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Mar 1, 2012·Chemistry and Physics of Lipids·Burkhard Bechinger, Evgeniy S Salnikov
Jul 4, 2007·Journal of Magnetic Resonance·Riqiang FuTimothy A Cross
Dec 19, 2006·Journal of Magnetic Resonance·Peter L Gor'kovWilliam W Brey
Sep 6, 2011·Biophysical Journal·Anna A De AngelisMyriam L Cotten
Sep 9, 2009·Biochimica Et Biophysica Acta·Eduard Y ChekmenevMyriam Cotten
Aug 19, 2014·Protein Expression and Purification·Wen Chen, Myriam L Cotten
Sep 23, 2016·Biophysical Journal·B Scott PerrinRichard W Pastor
Apr 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Hannah CetukSergei Sukharev
May 4, 2021·The Journal of Physical Chemistry Letters·Riqiang FuMyriam L Cotten
Oct 1, 2021·Journal of Fish Diseases·Benjamin S PriorBenjamin H Beck
Sep 2, 2020·Biochimica Et Biophysica Acta. Biomembranes·Ji-Ho JeongYongae Kim

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