High frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution.

Biomedical Optics Express
Brady HuntRebecca Richards-Kortum

Abstract

High-resolution microendoscopy (HRME) is a low-cost strategy to acquire images of intact tissue with subcellular resolution at frame rates ranging from 11 to 18 fps. Current HRME imaging strategies are limited by the small microendoscope field of view (∼0.5 mm2); multiple images must be acquired and reliably registered to assess large regions of clinical interest. Image mosaics have been assembled from co-registered frames of video acquired as a microendoscope is slowly moved across the tissue surface, but the slow frame rate of previous HRME systems made this approach impractical for acquiring quality mosaicked images from large regions of interest. Here, we present a novel video mosaicking microendoscope incorporating a high frame rate CMOS sensor and optical probe holder to enable high-speed, high quality interrogation of large tissue regions of interest. Microendoscopy videos acquired at >90 fps are assembled into an image mosaic. We assessed registration accuracy and image sharpness across the mosaic for images acquired with a handheld probe over a range of translational speeds. This high frame rate video mosaicking microendoscope enables in vivo probe translation at >15 millimeters per second while preserving high image q...Continue Reading

References

Dec 10, 2007·Optics Express·Timothy J MuldoonRebecca Richards-Kortum
Jan 21, 2011·Journal of Visualized Experiments : JoVE·Mark PierceRebecca Richards-Kortum
Mar 18, 2011·Head & Neck·Timothy J MuldoonAnn M Gillenwater
May 4, 2012·Cancer Prevention Research·Mark C PierceAnn M Gillenwater
Oct 20, 2012·Biomedical Optics Express·Noah BedardTomasz S Tkaczyk
Dec 27, 2012·IEEE Transactions on Bio-medical Engineering·Mustafa Suphi ErdenGuillaume Morel
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Apr 25, 2015·Biomedical Optics Express·Michael Hughes, Guang-Zhong Yang
Apr 25, 2018·International Journal of Computer Assisted Radiology and Surgery·Daniele RavìTom Vercauteren
Jul 11, 2019·Gynecologic Oncology·Sonia G ParraRebecca R Richards-Kortum
Aug 6, 2019·Frontiers in Oncology·Patra CharalampakiAxel Heimann
Feb 6, 2020·Biomedical Optics Express·Yubo TangRebecca R Richards-Kortum
Feb 27, 2020·Journal of Biomedical Optics·Andrew Thrapp, Michael Hughes
Apr 5, 2020·Journal of Biophotonics·Chengbo YinJonathan T C Liu
Jul 16, 2020·Optics Letters·Ziyi MengXin Yuan

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