High-frequency homologous recombination between duplicate chromosomal immunoglobulin mu heavy-chain constant regions.

Molecular and Cellular Biology
Mark D Baker

Abstract

Homologous recombination was used in a previous study to correct a 2-base-pair deletion in the third constant domain (Cmu3) of the haploid chromosomal mu gene in a mutant hybridoma cell line by transfer of a pSV2neo vector bearing a subfragment of the normal Cmu region (M.D. Baker, N. Pennell, L. Bosnoyan, and M.J. Shulman, Proc. Natl. Acad. Sci. USA 85:6432-6436, 1988). In these experiments, both gene replacement and single reciprocal crossover events were found to restore normal, cytolytic 2,4,6-trinitrophenyl-specific immunoglobulin M production to the mutant cells. In the cases of single reciprocal recombination, the structure of the recombinant mu gene is such that the normal Cmu region, in its correct position 3' of the expressed 2,4,6-trinitrophenyl-specific heavy-chain variable region, is separated from the mutant Cmu region by the integrated vector sequences. I report here that homologous recombination occurs with high frequency between the duplicate Cmu regions in mitotically growing hybridoma cells. The homologous recombination events were easily detected since they generated hybridomas that were phenotypically different from the parental cells. Analysis of the recombinant cells suggests that gene conversion is the m...Continue Reading

References

Feb 24, 1989·Cell·B J Thomas, R Rothstein
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M D BakerM J Shulman
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R M Liskay, J L Stachelek
Sep 1, 1986·Somatic Cell and Molecular Genetics·W S TrimbleM J Shulman
Jan 1, 1986·Annual Review of Immunology·G D Yancopoulos, F W Alt
Apr 1, 1985·Molecular and Cellular Biology·S Subramani, J Rubnitz
Jul 2, 1973·European Journal of Biochemistry·M Gross-BellardP Chambon
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jul 23, 1981·Nature·J A Jackson, G R Fink
Jan 1, 1981·Immunological Reviews·J M AdamsS Cory
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R G HawleyN Hozumi
Apr 14, 1983·Nature·S Tonegawa
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·A J Smith, P Berg
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·R M LiskayA Letsou
Jan 1, 1981·Immunological Reviews·T HonjoN Ishida
Sep 1, 1982·Molecular and Cellular Biology·M J ShulmanG Köhler
Jan 15, 1981·Nature·H L Klein, T D Petes

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Citations

Feb 12, 2008·Journal of Molecular Biology·Ania RukśćMark D Baker
Mar 1, 1995·European Journal of Immunology·C KardinalR Mocikat
Sep 1, 1990·Somatic Cell and Molecular Genetics·G M AdairK A Brotherman
Jul 1, 1993·Somatic Cell and Molecular Genetics·M D Baker, L R Read
Aug 12, 2008·Molecular Genetics and Genomics : MGG·Wen ZhangDacheng Tian
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven J RaynardMark D Baker
Dec 1, 1996·Molecular and Cellular Biology·M D BakerP Ng
May 1, 1991·Molecular and Cellular Biology·L H ReidO Smithies

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