High frequency of de novo DAZ microdeletion in sperm nuclei of subfertile men: possible involvement of genome instability in idiopathic male infertility

Human Fertility : Journal of the British Fertility Society
Hossein MozdaraniAnahita Mohseni-Meybodi

Abstract

The occurrence and diagnosis of Y-chromosome microdeletions, specifically deletions of the DAZ (Deleted in Azoospermia) genes are an important issue in male infertility. Screening Y chromosome microdeletion is mainly done using polymerase chain reaction (PCR) on blood leukocytes. However, there is some evidence indicating that presence of DAZ in somatic cells might not be indicative of its presence in the germ cell lineage. Therefore, a total of 130 men with poor semen quality were examined for presence of DAZ microdeletion in their leukocytes. From these, sperm from 40 randomly selected men with no DAZ microdeletions in their leukocytes (n = 10 oligozoospermia; n = 10 asthenozoospermia; n = 10 oligoasthenozoospermia; and n = 10 near-azoospermia) were were compared to sperm from men of normal semen quality (n = 10) using combined primed in situ labelling and fluorescent in situ hybridization (PRINS-FISH) technique as well as screening for sex chromosome aneuploidy. There was an increased frequency of DAZ microdeletion in blood samples from oligozoospermic (5%) (p < 0.05) and near azoospermic patients (14%) (p < 0.01). A high frequency of DAZ microdeletion was observed in the sperm of patients with no DAZ microdeletion in their ...Continue Reading

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Citations

Jan 19, 2019·Human Genetics·Rossella CannarellaAldo E Calogero
Feb 20, 2018·Reproductive Biology and Endocrinology : RB&E·Stacy Colaco, Deepak Modi
Oct 24, 2019·Human Fertility : Journal of the British Fertility Society·Jacob NethertonMark Baker
Dec 10, 2019·Science China. Life Sciences·Lu WenZi-Jiang Chen

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