High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

Animals : an Open Access Journal From MDPI
Kihae RaByeong Chun Lee

Abstract

Advanced maternal age (AMA) has become prevalent globally. With aging, weakened antioxidant defense causes loss of normal function in the ovary and uterus due to oxidative stress. Here, we aimed to improve embryo development in AMA mice by intravenous injection (IV) of human adipose stem cell conditioned medium (ASC-CM) at various frequencies and intervals as an antioxidant intervention. Four- and six-month-old female ICR (Institute of Cancer Research) mice were randomly divided into groups IV treated with human ASC-CM under different conditions, and in vitro and in vivo embryo development were evaluated. Consequently, compared to the control group, blastocyst formation rate of parthenotes was significantly promoted in 4-month-old mice and the mean number of implanted fetuses after natural mating was significantly increased by approximately two-fold in 6-month-old mice. Through gene analysis, the anti-apoptotic and anti-oxidative effects of human ASC-CMs were confirmed in the ovaries and uterus of pregnant mice at both ages. In particular, ovarian expression of gpx1 and catalase drastically increased in 6-month-old mice. Furthermore, the levels of gpx1 and catalase were further increased, with a high frequency of injection rega...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M NaritaY Tsujimoto
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Feb 20, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Olivier BaudPaul A Rosenberg
Oct 9, 2004·Journal of Animal Science·R L Krisher
Jun 4, 2005·Obstetrics and Gynecology·K S JosephRobert Liston
Jul 16, 2005·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalRakesh K Sharma
Jun 6, 2007·Human Reproduction Update·K DiedrichUNKNOWN Evian Annual Reproduction (EVAR) Workshop Group
Oct 22, 2008·Oncogene·J Li, J Yuan
Jun 16, 2009·Expert Opinion on Biological Therapy·Won-Serk KimJong-Hyuk Sung
Jul 8, 2009·Human Reproduction·Michal J SimchenEyal Schiff
Sep 24, 2009·Reproductive Biology and Endocrinology : RB&E·Carlo AlviggiStephen G Hillier
Dec 15, 2010·Biology of Reproduction·Jinhwan Lim, Ulrike Luderer
Jan 7, 2011·Molecular and Cellular Biochemistry·Mohammad Shamsul OlaHaseeb Ahsan
Jan 14, 2011·Fetal Diagnosis and Therapy·Juan Balasch, Eduard Gratacós
Nov 4, 2011·Human Reproduction·Yueping Alex WangElizabeth Anne Sullivan
May 2, 2012·Biochimica Et Biophysica Acta·Francesco BertoliniMikhail G Kolonin
Oct 30, 2012·Human Reproduction Update·S M NelsonR A Anderson
Jan 8, 2013·Molecular Aspects of Medicine·Shuang ZhangD Randall Armant
Aug 29, 2013·Journal of Assisted Reproduction and Genetics·Michaël De BruckerMichel Camus
May 8, 2014·BioMed Research International·Cheng PengZhen-Yu Chen
Dec 23, 2014·BioMed Research International·Jeanne Adiwinata Pawitan
May 3, 2016·Current Stem Cell Research & Therapy·Alexander Mildmay-White, Wasim Khan
Jun 25, 2016·International Journal of Molecular Sciences·Shan LiuYuanyuan Zhang
Nov 17, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Woong Chol KangKyunghee Byun
Apr 13, 2017·Laboratory animal research·Hye-Jun ShinMyeon-Woo Chung
Nov 18, 2017·Oxidative Medicine and Cellular Longevity·Shan WangXinghui Liu
Aug 14, 2018·Journal of Ovarian Research·Rani Fritz, Sangita Jindal
Aug 22, 2018·Reproductive Biology and Endocrinology : RB&E·Jiayin LuYulan Dong
Nov 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Izhar Hyder QaziGuang-Bin Zhou
Mar 27, 2020·Midwifery·Jennifer Nottingham-JonesTristan Leslie Snell

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

CellQuest Pro
GraphPad Prism

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