High frequency of LOH, MSI and abnormal expression of FHIT in gastric cancer

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
C HuipingS Ingvarsson

Abstract

The FHIT gene is a putative tumour suppressor gene. In this study, we analysed a set of 50 gastric tumours for alterations of FHIT, and found 38 of 45 tumours (84%) exhibiting loss of heterozygosity (LOH) within the FHIT gene. We used both nested Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and single step RT-PCR to analyse the FHIT transcripts and found 34 of 39 (87%) tumours and seven of the 11 (64%) corresponding non-cancerous tissues showed low or aberrant expression of FHIT mRNA and the appearance of the aberrant FHIT transcripts depended on the conditions of the RT-PCR. In these aberrant transcripts, frequent deletions and/or insertions were detected by direct sequencing. All breakpoints for deletions and insertions were at splicing sites. All insertions came from the adjacent introns, whose appearance was completely in accordance with the 'GU-AG' rule for pre-mRNA splicing. It may be suggested that an alternative splicing mechanism functions in the formation of these aberrant transcripts. The fragile nature of FRA3B within the FHIT gene could be responsible for the formation of the aberrant mRNA. Negative or reduced Fhit expression was detected in 39 of 50 tumours (78%). Moreover, an association was found bet...Continue Reading

References

Apr 5, 1996·Cell·G SozziC M Croce
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z SiprashviliK Huebner
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·H InoueC M Croce
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H C PaceC Brenner
Jun 13, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J T BergthorssonA Arason
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M GemmillH A Drabkin
Jan 14, 1999·Genes, Chromosomes & Cancer·Y TokuchiE Tsuchiya
Aug 14, 1999·The Journal of Biological Chemistry·A R ChaudhuriL D Barnes
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Y FongK Huebner
Aug 10, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C HuipingS Ingvarsson
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K R DumonC M Croce
Sep 20, 2001·Seminars in Cancer Biology·S Ingvarsson

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Citations

May 22, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yasuhiko KimuraShigeo Yokoyama
Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sandra G DurkinThomas W Glover
Nov 8, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E BriaA Scarpa
Jan 9, 2013·Human Molecular Genetics·Laura W DillonYuh-Hwa Wang
Oct 31, 2012·International Journal of Molecular Sciences·Ke MaZheng Li
Feb 1, 2007·Frontiers of Medicine in China·Yuping XiaoYan Xin
Oct 12, 2005·Journal of Cancer Research and Clinical Oncology·Emma BragantiniAldo Scarpa
May 23, 2015·Oncology Reports·Sebastian MayerElmar Stickeler
Feb 4, 2010·Biochimica Et Biophysica Acta·Ana Manuel Dantas MachadoLene Juel Rasmussen
Jun 5, 2004·Biochemical and Biophysical Research Communications·Samuel KoJohn Wong
Jan 14, 2005·Genes, Chromosomes & Cancer·Zane KalninaAija Linē
Jul 16, 2004·Journal of Cellular Physiology·Manuela MigliavaccaAntonio Russo
Dec 17, 2003·Journal of Gastroenterology and Hepatology·Kevin DolanRobert Sutton
Oct 9, 2015·BioMed Research International·Ning Yi YapGlenda Carolyn Gobe
Nov 11, 2008·Biochimica Et Biophysica Acta·Jamal TaziStefan Stamm
May 29, 2003·British Journal of Cancer·K Huebner, C M Croce
Jun 2, 2005·World Journal of Gastroenterology : WJG·Yu-Ping XiaoYan Xin
Mar 9, 2018·Asia-Pacific Journal of Clinical Oncology·Lisiane Silveira ZavalhiaAdriana Vial Roehe
Jun 15, 2016·World Journal of Gastroenterology : WJG·Tian-Hui ZouJing-Yuan Fang
Jun 15, 2006·World Journal of Gastroenterology : WJG·Yu-Ping XiaoYan Xin
May 8, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Seiji SuzukiTakashi Tajiri

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