PMID: 8600570Nov 1, 1995Paper

High frequency recombination between loxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase

Somatic Cell and Molecular Genetics
P WangS Bacchetti

Abstract

An adenovirus vector (AdCre1) expressing Cre recombinase has been used to induce recombination between loxP sites in human chromosomes. G418 resistant cells with one loxP site, generated by transfection with a plasmid containing loXp between the SV40 promoter and the G418 resistance (neo) gene, were infected with AdCre1 and transfected with a plasmid containing loxP adjacent to a promoterless hisD gene. This resulted in integration of hisD downstream of the SV40 promoter with gain of histidinol and loss of G418 resistance. Since AdCre1 is non-replicating and Cre expression transient, histidinol resistant cells containing the hisD gene flanked by loxP sites were stable. Reinfection of these cells with AdCre1 induced excision of hisD in over 90% of infected cells. This high efficiency of site-specific recombination suggests that AdCre1 may be exploited for temporal and tissue-specific regulation of gene expression and for chromosome engineering in vitro and in animals.

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Citations

Dec 22, 2006·Molecular Biology of the Cell·Kongming WuRichard G Pestell
Oct 12, 2010·Molecular Biology of the Cell·Sanjay KatiyarRichard G Pestell
Jun 3, 2014·Biological & Pharmaceutical Bulletin·Yuto SakaiHidetoshi Hayashi
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christine VoelkelChristopher Baum
Jan 14, 1998·Gene·N SatoR W Oppenheim
Jan 7, 2004·Drug Metabolism Reviews·Colin J HendersonC Roland Wolf
May 6, 2009·Journal of Molecular Biology·Saki KondoYumi Kanegae
Mar 11, 2008·Cancer Cell·Johan JongsmaAnton Berns
Sep 1, 2008·Clinical and Translational Science·Jaime LindsayRichard G Pestell
Jun 3, 1998·Methods : a Companion to Methods in Enzymology·B Sauer
Mar 26, 1998·Biochemical and Biophysical Research Communications·Y SatoI Saito

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