PMID: 375221Mar 1, 1979Paper

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules

Proceedings of the National Academy of Sciences of the United States of America
K StruhlR W Davis

Abstract

A set of vector DNAs (Y vectors) useful for the cloning of DNA fragments in Saccharomyces cerevisiae (yeast) and in Escherichia coli are characterized. With these vectors, three modes of yeast transformation are defined. (i) Vectors containing yeast chromosomal DNA sequences (YIp1, YIp5) transform yeast cells at low frequency (1--10 colonies per microgram) and integrate into the genome by homologous recombination; this recombination is reversible. (ii) Hybrids containing endogenous yeast plasmid DNA sequences (YEp2, YEp6) transform yeast cells at much higher frequency (5000--20,000 colonies per microgram). Such molecules replicate autonomously with an average copy number of 5--10 covalently closed circles per yeast cell and also replicate as a chromosomally integrated structure. This DNA may be physically isolated in intact form from either yeast or E. coli and used to transform either organism at high frequency. (iii) Vectors containing a 1.4-kilobase yeast DNA fragment that includes the centromere linked trp1 gene (YRp7) transform yeast with an efficiency of 500--5000 colonies per microgram; such molecules behave as minichromosomes because they replicate autonomously but do not integrate into the genome. The uses of Y vectors...Continue Reading

References

Jan 7, 1977·Molecular & General Genetics : MGG·N E MurrayK Murray
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Ratzkin, J Carbon
Jan 1, 1977·Nucleic Acids Research·J R CameronR W Davis
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Struhl, R W Davis
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G D Clark-Walker

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Citations

Jan 1, 1997·Journal of Basic Microbiology·F HänelT Munder
Feb 8, 2002·Archives of Biochemistry and Biophysics·Ewa GrzybowskaJoanna Rytka
May 1, 1989·Plant Molecular Biology·T T EckdahlJ N Anderson
Feb 1, 1991·Molecular & General Genetics : MGG·J ArnauP Strøman
Jan 1, 1981·Molecular & General Genetics : MGG·J A GormanN Warren
Aug 1, 1993·Molecular & General Genetics : MGG·C Fairhead, B Dujon
Sep 1, 1980·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·V N Soyfer
Jan 1, 1987·Molecular & General Genetics : MGG·R Rodicio, J Heinisch
Dec 1, 1987·Molecular & General Genetics : MGG·S E Kearsey, J Edwards
Jan 1, 1985·Molecular & General Genetics : MGG·S HarashimaY Oshima
Jan 1, 1983·Molecular & General Genetics : MGG·H D SchmittF K Zimmermann
Jun 1, 1987·Molecular & General Genetics : MGG·K NishiwakiY Oshima
Jan 1, 1986·Molecular & General Genetics : MGG·A Aguilera, F K Zimmermann
Jan 1, 1983·Molecular & General Genetics : MGG·H Eibel, P Philippsen

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