High glucose and diabetes increase the release of [3H]-D-aspartate in retinal cell cultures and in rat retinas

Neurochemistry International
A R SantiagoA F Ambrósio

Abstract

Several evidences suggest that glutamate may be involved in retinal neurodegeneration in diabetic retinopathy (DR). For that reason, we investigated whether high glucose or diabetes affect the accumulation and the release of [(3)H]-D-aspartate, which was used as a marker of the glutamate transmitter pool. The accumulation of [(3)H]-D-aspartate did not change in cultured retinal neural cells treated with high glucose (30 mM) for 7 days. However, the release of [(3)H]-D-aspartate, evoked by 50 mM KCl, significantly increased in retinal cells exposed to high glucose. Mannitol, which was used as an osmotic control, did not cause any significant changes in both accumulation and release of [(3)H]-D-aspartate. In the retinas, 1 week after the onset of diabetes, both the accumulation and release of [(3)H]-D-aspartate were unchanged comparing to the retinas of age-matched controls. However, after 4 weeks of diabetes, the accumulation of [(3)H]-D-aspartate in diabetic retinas decreased and the release of [(3)H]-D-aspartate increased, compared to age-matched control retinas. These results suggest that high glucose and diabetes increase the evoked release of D-aspartate in the retina, which may be correlated with the hypothesis of glutamat...Continue Reading

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Citations

Jul 11, 2013·PloS One·Joana VindeirinhoPaulo F Santos
Apr 24, 2012·Molecular and Cellular Neurosciences·G N CostaP F Santos
Apr 2, 2016·Journal of Diabetes Research·J VindeirinhoP F Santos
Mar 3, 2010·Experimental Eye Research·Wei ZhengLuo Lu
Jul 31, 2007·Experimental Eye Research·David J RamseyHaohua Qian
Mar 14, 2007·Neurochemistry International·Ana Rita AlvaroCláudia Cavadas
Apr 7, 2009·Journal of Neurochemistry·Ana Rita AlvaroCláudia Cavadas
Dec 17, 2008·Journal of Neuroscience Research·G CostaP F Santos
Mar 12, 2008·Journal of Neurochemistry·Ana Rita AlvaroCláudia Cavadas
Dec 26, 2009·Brain Research·Tiago de Oliveira Simões PereiraPaulo Fernando Martins dos Santos
Jul 30, 2014·Experimental Eye Research·Filipa I BaptistaAntónio F Ambrósio
Jan 24, 2007·Neurobiology of Disease·A R SantiagoA F Ambrósio
Oct 21, 2010·Current Eye Research·A R SantiagoA F Ambrósio
Jul 31, 2018·Frontiers in Physiology·Ana R SantiagoRosa Fernandes
Feb 29, 2020·Pharmacology & Therapeutics·Ana Raquel SantiagoAntónio Francisco Ambrósio

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