High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression.

The Journal of Obstetrics and Gynaecology Research
Jun TaoZuo Wang

Abstract

This study aimed to investigate the effect of high glucose (HG) level on the proliferation, migration and invasion of trophoblasts and determine the role of placental growth factor (PLGF) in the process. HTR8-S/Vneo was treated with different concentrations of d-glucose (0, 10, 15, 20, 25 and 30 μM) at different times (0, 6, 12 and 24 h). qRT-PCR and Western blot analyses were used to measure PLGF expression. The protein level of PLGF was measured by immunofluorescence. Cell proliferation was assessed with CCK-8 analysis. Wound healing and transwell assays were used to evaluate cell migration and invasion. Intercellular ROS was detected with DCFH-DA. After d-glucose treatment, the viability decreased in 25 and 30 μM groups. The HG group (25 μM) showed inhibited cell migration and invasion ability. The mRNA and protein levels of PLGF decreased under HG condition. Elevated ROS production was also detected in the HG group. Knocked-down PLGF expression enhanced increased ROS production and decreased cell migration and invasion, which reverted to the original levels after PLGF was overexpressed. High glucose treatment inhibited HTR8-S/Vneo viability, migration and invasion by downregulating PLGF expression.

References

Feb 7, 2004·The New England Journal of Medicine·Richard J LevineS Ananth Karumanchi
May 11, 2005·Kidney International·S Ananth KarumanchiVikas P Sukhatme
Oct 21, 2005·Reproductive Biology and Endocrinology : RB&E·Elsebeth Staun-Ram, Eliezer Shalev
Feb 10, 2007·Reproductive Biology and Endocrinology : RB&E·Laura LunghiFortunato Vesce
May 15, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jean-Paul Duong Van HuyenMartine Lelièvre-Pégorier
Mar 21, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Oksana ShynlovaStephen J Lye
Jul 14, 2009·Journal of Perinatal Medicine·Chadakarn Phaloprakarn, Siriwan Tangjitgamol
Aug 24, 2010·Human Reproduction Update·Hakan Cakmak, Hugh S Taylor
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Keiichi KumasawaMasaru Okabe
Jun 4, 2011·Diabetes Care·Valerie A HolmesUNKNOWN Diabetes and Pre-eclampsia Intervention Trial Study Group
May 24, 2012·Obstetrics and Gynecology·Marianne CoolmanEric A P Steegers
Jul 1, 2012·Pregnancy Hypertension·K A NerenbergP Kaul
Sep 27, 2015·Journal of Physiology and Biochemistry·Stella Liong, Martha Lappas
Nov 17, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Aleksandra NikolicZoran Stosic
Nov 22, 2015·American Journal of Reproductive Immunology : AJRI·Fan WuWang-Ming Xu
Mar 31, 2016·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Irene CetinPatrizia Vergani
Apr 21, 2016·BMJ : British Medical Journal·Emily BartschUNKNOWN High Risk of Pre-eclampsia Identification Group
Jun 18, 2016·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·P J CorreaS E Illanes
Aug 4, 2016·Molecular Medicine Reports·Rong-Rong XuanHai-Min Chen
Oct 25, 2016·Methods : a Companion to Methods in Enzymology·Elisa CarrascoJesús Espada
Apr 7, 2017·Current Drug Targets·Xuejing SongWei Long
Jul 18, 2017·Seminars in Nephrology·Belinda Jim, S Ananth Karumanchi
Nov 10, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Siqi XuHanshi Xu
Feb 3, 2018·Molecular Reproduction and Development·Alejandra Sánchez-SantosLuis A Baiza-Gutman
Jun 2, 2018·American Journal of Reproductive Immunology : AJRI·Si-Ming ZhangYi Lin
Sep 4, 2018·American Journal of Reproductive Immunology : AJRI·Kathleen R HeimVikki M Abrahams
Nov 6, 2018·Progress in Retinal and Eye Research·Tine Van BergenAlan W Stitt
Jan 19, 2019·Postepy biochemii·Anna Filipek, Ewelina Jurewicz
Jul 2, 2019·The Journal of Obstetrics and Gynaecology Research·Kewei ZhangThushari I Alahakoon

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