High glucose increases mesangial lipid accumulation via impaired cholesterol transporters

Transplantation Proceedings
K H SongH Ha

Abstract

Diabetes, whether it occurs before or after transplantation, plays an important role to decrease graft function and survival. In addition renal lipid accumulation has been suggested to play a role in the development and progression of chronic renal allograft rejection. Intracellular lipid accumulation is governed by a balance between the influx and efflux of lipid. Cholesterol transporters, such as scavenger receptor (SR)-A1, CD36, and ATP binding cassette (ABC) A1 and G1 (ABCG1), coordinate to regulate cellular lipid status. Therefore, in the present study, we examined whether high glucose caused lipid accumulation in mesangial cells as a result of altered cholesterol transporters. Mouse mesangial cells were stimulated with 30 mmol/L D-glucose (high glucose); 100 μmol/L oleic acid (OA) used as a positive control. Cellular lipid accumulation was measured by Oil Red O staining. Protein and mRNA expression of cholesterol influx (SR-A1 and CD36) and efflux (ABCA1 and ABCG1) transporters were evaluated using Western blot analysis and real-time quantitative polymerase chain reaction, respectively. High glucose was shown to significantly increase lipid accumulation in mesangial cells at 24 hours as was observed for OA. SR-A1 and CD36...Continue Reading

References

Jan 7, 2004·American Journal of Nephrology·Christine K Abrass
Dec 8, 2009·Journal of Lipid Research·Chongren TangJohn F Oram
Dec 1, 2010·Kidney International. Supplement·Esteban PorriniArmando Torres
Jan 5, 2011·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Alan A Wanderer
Feb 12, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jehyun ParkHunjoo Ha

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Citations

Sep 3, 2014·PloS One·Joseph G S TsunKathryn C B Tan
Sep 19, 2017·Nature Reviews. Nephrology·Xiaochun YangXiong Z Ruan
Apr 30, 2015·American Journal of Physiology. Endocrinology and Metabolism·Yang ZhangBi Cheng Liu

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