High glucose increases the expression of proinflammatory cytokines and secretion of TNFα and β-hexosaminidase in human mast cells

European Journal of Pharmacology
Kanji NagaiMasuko Kobori

Abstract

Epidemiological studies demonstrated that obesity, which is a high-risk factor for development of hyperglycemia-associated metabolic syndromes, is associated with prevalence/incidence of allergic diseases. To elucidate the underlying mechanisms of the relationship between hyperglycemia and allergy, we examined the effect of high glucose on the activation of human mast cell lines, HMC-1 and LAD2. HMC-1 and LAD2 cells were cultured in low (5.5 mM) and high (25 mM)-glucose Dulbecco's modified Eagle's medium (DMEM). High-glucose medium increased the intracellular reactive oxygen species levels in HMC-1 and LAD2 cells after 2 days of incubation; in HMC-1 cells, the expression levels of tumor necrosis factor (TNF) α, interleukin (IL)-1β, IL-6, and IL-13 were increased significantly. The β-hexosaminidase release rates were not significantly different between LAD2 cells cultured in both media; however, the intracellular and extracellular activities of β-hexosaminidase in cells were significantly higher in high-glucose than in low-glucose media. High glucose increased the secretion of TNFα by unstimulated HMC-1 cells and IgE crosslinking-stimulated LAD2 cells. High glucose increased the phosphorylation of extracellular signal-regulated ...Continue Reading

References

Jan 1, 1988·Leukemia Research·J H ButterfieldG J Gleich
May 1, 1994·Scandinavian Journal of Immunology·G NilssonL Hellman
Dec 10, 1999·Nature·S T Holgate
Jul 29, 2003·European Journal of Immunology·Barbara FrossiCarlo Pucillo
Jul 2, 2004·The Journal of Biological Chemistry·Silvia MonticelliAnjana Rao
May 4, 2005·The Journal of Allergy and Clinical Immunology·Earl S Ford
Feb 24, 2006·Toxicology and Applied Pharmacology·Anabela P Rolo, Carlos M Palmeira
Jan 29, 2008·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Takashi KusunokiTatsutoshi Nakahata
Apr 10, 2008·The British Journal of Nutrition·Paul TrayhurnI Stuart Wood
Apr 15, 2008·The Journal of Allergy and Clinical Immunology·Stephanie A Shore
Oct 1, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Luiz Fernando de SouzaElena Aida Bernard
Oct 22, 2008·Nature Immunology·Janet Kalesnikoff, Stephen J Galli
May 15, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yael Minai-Fleminger, Francesca Levi-Schaffer
Jun 23, 2009·Diabetes & Metabolism·V Bourlier, A Bouloumie
Dec 22, 2009·Nature Immunology·Rongbin ZhouJürg Tschopp
Jun 19, 2010·International Archives of Allergy and Immunology·Yue ChenJames Dosman
Dec 18, 2010·Free Radical Biology & Medicine·Justin L Rains, Sushil K Jain
Dec 28, 2010·Biochimica Et Biophysica Acta·Jie Zhang, Guo-Ping Shi

❮ Previous
Next ❯

Citations

Mar 4, 2014·The Journal of Allergy and Clinical Immunology·Tal Hadad ErlichEhud Razin
Jul 6, 2019·European Journal of Immunology·Julia Matthias, Christina E Zielinski
Sep 5, 2015·Current Opinion in Lipidology·Miriam Lee-Rueckert, Petri T Kovanen
Oct 25, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenshiro MatsudaHiroshi Matsuda
Dec 31, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Izabela Tuleta, Nikolaos G Frangogiannis
Aug 1, 2021·Advanced Drug Delivery Reviews·Izabela Tuleta, Nikolaos G Frangogiannis

❮ Previous
Next ❯

Related Concepts

Related Feeds

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.