High glucose-induced reactive oxygen species generation promotes stemness in human adipose-derived stem cells

Cytotherapy
Nai-Chen ChengTai-Horng Young

Abstract

Adipose-derived stem cells (ASCs) represent an important source of cell therapy to treat diabetic complications. However, hyperglycemia may alter several cellular functions, so the present study aimed to investigate the influence of a diabetic environment on the stemness and differentiation capabilities of ASCs. Human ASCs were obtained from subcutaneous adipose tissues of diabetic (dASCs) and nondiabetic donors (nASCs) and characterized. To reproduce an in vitro hyperglycemia environment, the nASCs were also cultured under prolonged high-glucose (HG; 4.5 g/L) or low-glucose (LG; 1.0 g/L) conditions. The expression of cell surface markers in dASCs and nASC was similar and characteristic of mesenchymal stem cells. Although dASCs or HG-treated nASCs exhibited decreased proliferation, enhanced expression of the pluripotent markers Sox-2, Oct-4, and Nanog was observed. Moreover, HG-treated nASCs exhibited decreased cell migration, enhanced senescence, and significantly higher intracellular reactive oxygen species (ROS), whereas their adipogenic and osteogenic differentiation capacities remained comparable to LG-treated cells. With antioxidant treatment, HG-treated nASCs showed improved cell proliferative activity without stemness e...Continue Reading

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Citations

May 5, 2017·International Journal of Molecular Sciences·Alexander Sorisky
Jan 10, 2020·Journal of Cellular and Molecular Medicine·Bingru ZhouPing Zou
May 10, 2020·Cells·Vineet Kumar MishraLiang-Yin Ke
Jul 4, 2020·Molecular Medicine·Aleksandra Skubis-SikoraJoanna Gola
Jun 29, 2017·Molecular Medicine Reports·Dayong ZhangJianping Pan
Apr 8, 2020·International Journal of Molecular Sciences·Féaron C CassidyCynthia M Coleman
May 11, 2021·Frontiers in Cell and Developmental Biology·Min YinXia Li
May 28, 2021·Journal of Theoretical Biology·Tagari Samanta, Sandip Kar
Aug 30, 2021·The International Journal of Biochemistry & Cell Biology·Nourhan Abu-ShahbaOsama Azmy

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