PMID: 8603769Apr 1, 1996Paper

High glucose induces antioxidant enzymes in human endothelial cells in culture. Evidence linking hyperglycemia and oxidative stress

Diabetes
A CerielloP Cerutti

Abstract

It has been suggested that oxidative stress may play an important role in the pathogenesis of diabetic complications. Hyperglycemia may cause increased production of free radicals, and evidence supports a prominent role for these reactive molecules as mediators of endothelial cell dysfunction in diabetes. It has been demonstrated that active oxygen species induce antioxidant enzyme expression in some tissues, and this phenomenon is considered proof of an existing oxygen-dependent toxicity. In this study, human endothelial cells from umbilical vein, immortalized human endothelial cells, and immortalized human endothelial cells transfected to express high glutathione peroxidase levels were grown in normal and high-glucose conditions. High glucose delayed replication after 7 and 14 days of culture of human endothelial cells, both from umbilical vein and immortalized, while transfected cells were not affected. The activity and the mRNA expression of the antioxidant enzymes CuZn-superoxide-dismutase, Mn-superoxide-dismutase, catalase, and glutathione peroxidase were evaluated after 2, 7, and 14 days of culture. High glucose at days 7 and 14 induced an overexpression of CuZn-superoxide-dismutase, catalase, and glutathione peroxidase ...Continue Reading

Citations

Jul 22, 2005·Current Diabetes Reports·Xinhua Chen, Theresa O Scholl
Sep 7, 2001·Biochemical Pharmacology·J M MayX Li
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jan SkrhaHana Weiserová
Nov 14, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·D S ErdinçlerG Candan
Jul 13, 1999·The Journal of Laboratory and Clinical Medicine·W A Oranje, B H Wolffenbuttel
Sep 18, 1998·Diabetes Research and Clinical Practice·H Haller
Mar 30, 2002·General Pharmacology·D W LaightM J Carrier
Aug 28, 2001·Journal of Diabetes and Its Complications·N A AbdellaM A Moussa
Oct 17, 2002·Nitric Oxide : Biology and Chemistry·I NoymanZ Madar
Nov 13, 2008·Cytotechnology·Kiichiro TeruyaSanetaka Shirahata
Apr 28, 2000·Diabetic Medicine : a Journal of the British Diabetic Association·I C West
Feb 19, 2002·Kidney International·Mark A CatherwoodElisabeth R Trimble
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoshito TanakaR Paul Robertson
Jan 22, 2002·The Journal of Cardiovascular Nursing·Sue PenckoferKristine Florczak
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Emmanuelle MeugnierHubert Vidal
Jun 25, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Jason M NgBret H Goodpaster
Oct 16, 2004·The American Journal of Chinese Medicine·Eun Ju ChoJong Cheol Park
May 2, 2012·Cardiovascular Diabetology·Zhao Zhong Chong, Kenneth Maiese
Jul 6, 2012·Diabetology & Metabolic Syndrome·Mohamed T Abdel AzizHeba M Morsi
May 3, 2000·Cell Structure and Function·M IijimaH Urushihara
Feb 3, 2009·Biological & Pharmaceutical Bulletin·Masahiro NomotoYasushi Yamazoe
May 15, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Mariapina PomilioFrancesco Chiarelli
Jun 23, 2010·AJR. American Journal of Roentgenology·David H O'DonnellMichael J Lee
Jul 1, 2008·Oxidative Medicine and Cellular Longevity·Kenneth MaieseJinling Hou
Dec 1, 2001·Biochemical and Biophysical Research Communications·K SasakiN Makino
Nov 19, 1997·Journal of Endocrinological Investigation·D KonukoğluT Akçay
Jul 9, 2014·Pharmacology & Therapeutics·Giacinta GuariniMario Marzilli

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