High glucose inhibits human epidermal keratinocyte proliferation for cellular studies on diabetes mellitus.

International Wound Journal
Hiroto TerashiC L Marcelo

Abstract

In order to more clarify the delayed wound healing in diabetes mellitus, we cultured the human epidermal keratinocytes in both 6 mM (control group) and 12 mM glucose (high-glucose group) of "complete" MCDB 153 medium. Hyperglycaemia slowed the rate of their proliferation and inhibited their DNA synthesis and the production of total proteins. By 1 month after primary seeding in high-glucose group, the cells ceased their proliferation, whereas the cells in control group grew for more than 40 days. Mean population doublings in high-glucose group was 5.27 (vs. 7.25 in control, P = 0.001), and mean population doubling time during 1 month in high glucose group was 5.43 days (vs. 3.65 days in control, P = 0.02). They indicate that prolonged exposure to high glucose decreases the replicative life span of human epidermal keratinocytes in vitro. Furthermore, analysis of fatty acid contents in membrane phospholipids with thin-layer and gas chromatography showed no difference between the cultured keratinocytes in both conditions. Immunocytochemical staining of glucose transporter 1 shows that 28.1% of cells in high-glucose group were almost twice positive of those in control group (13.2%, P = 0.008). The mechanism of the ill effects of hig...Continue Reading

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Citations

Mar 17, 2010·Archives of Dermatological Research·Alexandra L Varol, Chris D Anderson
Jul 17, 2010·Journal of Burn Care & Research : Official Publication of the American Burn Association·Ricki Y FramDavid N Herndon
Mar 14, 2013·BioMed Research International·Jorge Berlanga-AcostaLuis Herrera-Martínez
Dec 1, 2012·Stem Cells Translational Medicine·Laura Shin, Daniel A Peterson
Oct 23, 2012·Hospital Practice·Andrea D MaderalRobert S Kirsner
Mar 16, 2013·Experimental Dermatology·Lucía Martínez-SantamaríaMarcela Del Río
Dec 12, 2013·The Journal of Investigative Dermatology·Xiao-Qi WangAmy S Paller
Jun 24, 2011·The Journal of Investigative Dermatology·Andrea L ChristmanLuis A Garza
Mar 20, 2009·The Journal of Investigative Dermatology·Giorgio PietramaggioriAmy J Wagers
Mar 12, 2015·Tissue Engineering. Part C, Methods·Hiroko KatoStephen E Feinberg
Feb 18, 2017·Journal of Cellular and Molecular Medicine·Dezhi ChenAixi Yu
Sep 15, 2017·Diabetic Foot & Ankle·Estrella Cervantes-García, Paz María Salazar-Schettino
May 1, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Francesca CianfaraniTeresa Odorisio
Jun 13, 2019·Journal of Aging and Physical Activity·Werner F HelsenAndrew M Williams
Oct 4, 2019·PloS One·Masafumi Aoki, Takatoshi Murase
Jul 24, 2020·Scientific Reports·Nsrein AliSeppo J Vainio
Jan 23, 2009·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Ailish BreenA Pandit
Apr 14, 2017·Dermato-endocrinology·Alberto Leguina-Ruzzi, Juan P Valderas

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