High glucose levels enhance platelet activation: involvement of multiple mechanisms

British Journal of Haematology
Dzana SudicN Li

Abstract

Diabetes mellitus (DM) and hyperglycaemia are associated with platelet activation. The present study was designed to investigate how high glucose levels influence platelet function. Fasting human blood was incubated with different concentrations of D-glucose (5, 15 and 30 mmol/l) and other sugars without or with in vitro stimuli. Platelet activation was monitored by whole blood flow cytometry. High glucose levels enhanced adenosine diphosphate (ADP)- and thrombin receptor-activating peptide (TRAP)-induced platelet P-selectin expression, and TRAP-induced platelet fibrinogen binding. Similar effects were seen with 30 mmol/l L-glucose, sucrose and galactose. Hyperglycaemia also increased TRAP-induced platelet-leucocyte aggregation. Protein kinase C (PKC) blockade did not counteract the enhancement of platelet P-selectin expression, but abolished the enhancement of TRAP-induced platelet fibrinogen binding by hyperglycaemia. Superoxide anion scavenging by superoxide dismutase (SOD) attenuated the hyperglycaemic enhancement of platelet P-selectin expression, but did not counteract the enhancement of TRAP-induced platelet fibrinogen binding. Hyperglycaemia did not alter platelet intracellular calcium responses to agonist stimulation. ...Continue Reading

References

Jun 21, 1990·The New England Journal of Medicine·G DavìC Patrono
May 19, 1988·The New England Journal of Medicine·M BrownleeH Vlassara
Jul 1, 1995·Diabetic Medicine : a Journal of the British Diabetic Association·M J StevensD A Greene
Oct 1, 1996·Annals of Medicine·H Vlassara
Oct 1, 1996·Coronary Artery Disease·H Miettinen, V Salomaa
Dec 17, 1997·Clinical Biochemistry·L Mazzanti, B Mutus
Jan 31, 1998·British Journal of Haematology·N LiP Hjemdahl
Apr 17, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·M YngenN H Wallén
Jul 27, 2001·Diabetes Care·A I VinikG L Pittenger
May 22, 2002·JAMA : the Journal of the American Medical Association·Joshua A BeckmanPeter Libby
Jul 2, 2003·Diabetes Care·John A Colwell, Richard W Nesto
Jan 14, 2004·Journal of Thrombosis and Haemostasis : JTH·H HuP Hjemdahl
Aug 12, 2004·Journal of Thrombosis and Haemostasis : JTH·P FerroniG Davì
Jan 26, 2006·Diabetic Medicine : a Journal of the British Diabetic Association·M YngenN H Wallén

❮ Previous
Next ❯

Citations

Oct 8, 2011·American Journal of Respiratory Cell and Molecular Biology·Damien J LaparVictor E Laubach
Aug 9, 2011·Vascular Health and Risk Management·William B HillegassGilbert J Zoghbi
Jan 20, 2010·Journal of Postgraduate Medicine·A Cengel, A Tanindi
Jan 9, 2016·Islets·Sonja HänzelmannAnders H Rosengren
Jul 10, 2012·Neurologia i neurochirurgia polska·Maria Lukasik, Wojciech Kozubski
Jan 19, 2011·Cardiovascular Revascularization Medicine : Including Molecular Interventions·Mickey ScheinowitzRon Waksman
Jun 24, 2008·Thrombosis Research·Yao-Zu XiangBo-Li Zhang
Sep 22, 2007·Thrombosis Research·Xiao Min ZhaoPhilip G de Groot
Jan 30, 2009·Journal of Thrombosis and Haemostasis : JTH·L M SteffenA R Folsom
Aug 6, 2013·Platelets·Gennadi KobzarNigulas Samel
Jan 31, 2015·Journal of Thrombosis and Thrombolysis·Masafumi UenoShunichi Miyazaki
Feb 6, 2017·International Journal of Food Sciences and Nutrition·Nevena KardumMaria Glibetić
Dec 26, 2009·European Journal of Anaesthesiology·Eliyahu ZismanReuven Pizov
Aug 7, 2008·The Journal of Biological Chemistry·Frederick A OfosuJohn W Fenton
Dec 15, 2015·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Bekir S YildizHarun Evrengul
Aug 19, 2017·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Tomasz RusakMarian Tomasiak
Oct 13, 2018·European Cardiology·Ricardo A Perez de la HozAlejandra Silvia Scazziota
Feb 10, 2020·Sleep & Breathing = Schlaf & Atmung·Ana C KriegerJoan H F Drosopoulos
Sep 10, 2020·Current Anesthesiology Reports·Ingrid Moreno-Duarte, Kamrouz Ghadimi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.