High glucose potentiates collagen synthesis and bone morphogenetic protein-2-induced early osteoblast gene expression in rat spinal ligament cells

Endocrinology
Hai LiLi-Yang Dai

Abstract

Type 2 diabetes mellitus (T2DM) is an independent risk factor for ossification of the posterior longitudinal ligament, but the mechanism is unclear. We isolated cells from rat cervical spine ligaments and studied the effects of high glucose on expression of osteoblast genes to provide insight into molecular mechanism. Using these cells, high glucose stimulated the synthesis of type I collagen and significantly potentiated expression of early osteoblast genes (Runx2; alkaline phosphatase, ALP; and osteopontin, OP) induced by bone morphogenetic protein-2 (BMP-2). Notably, these effects of high glucose were fully mimicked and augmented by H(2)O(2), although blocked by the reactive oxygen species inhibitor N-acetyl cysteine. Furthermore, exposure of these cells to high glucose significantly suppressed the phosphorylation of p38MAPK while enhancing the phosphorylation of protein kinase C (PKC) in the cells. Consistent with these observations, an inhibitor of p38 augmented the potentiation of high glucose on BMP-2-induced early osteogenic gene expression, whereas the PKC inhibitor repressed the effect of high glucose on type I collagen synthesis of the cells. In conclusion, high glucose, via production of reactive oxygen species, sub...Continue Reading

References

Mar 1, 1992·The Journal of Bone and Joint Surgery. British Volume·S MiyamotoK Ono
Jul 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M InabaH Morii
Jun 28, 1996·The Journal of Biological Chemistry·Y Y LoT F Cruz
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F GoriB L Riggs
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Ducy
Feb 13, 2001·Bone·E BalintS M Sprague
Aug 11, 2001·Free Radical Biology & Medicine·N ModyL L Demer
Jan 24, 2002·Spine·Nancy E EpsteinArnold S Breitbart
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 20, 2003·Journal of the American Society of Nephrology : JASN·Daniela VerzolaGiacomo Deferrari
Jan 13, 2004·Biochemical and Biophysical Research Communications·Xiao-chun BaiShen-qiu Luo
Feb 26, 2004·The American Journal of Pathology·H Clarke AndersonRama Dhamyamraju
Apr 3, 2004·Molecular and Cellular Biology·Tatsuya YoshizawaHiroyuki Kawashima
Apr 24, 2004·Spine·Gen KobashiUNKNOWN Japan Collaborative Epidemiological Study Group for Evaluation of Ossification of the Posterior Longitudinal Ligament of the
Oct 14, 2004·Journal of Epidemiology·Masakazu WashioUNKNOWN Japan Collaborative Epidemiological Study Group for Evaluation of Ossification of the Posterior Longitudinal Ligament of thr
Oct 19, 2004·Journal of Bone and Mineral Metabolism·Kazushi OkamotoUNKNOWN Japan Collaborative Epidemiological Study Group for Evaluation of Ossification of the Posterior Longitudinal Ligament of the
Feb 8, 2005·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Norihiko SakaiHitoshi Yokoyama
Feb 26, 2005·The Journal of Biological Chemistry·Xiao-chun BaiShen-qiu Luo
May 17, 2005·Calcified Tissue International·N YamamotoC Hamanishi
Sep 29, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Neal X ChenSharon M Moe
Nov 14, 2006·European Journal of Pharmacology·Alexander RiadCarsten Tschöpe
Apr 12, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Hai LiLi-Yang Dai
Jun 20, 2007·The Journal of Experimental Medicine·Kozo MoritaToshio Suda
Aug 4, 2007·Kidney International. Supplement·H B LeeH Ha
Aug 19, 2007·The Journal of Biological Chemistry·Dongwei FanYongfeng Shang
Sep 18, 2007·Biochemical and Biophysical Research Communications·Yu-Ming LiRegina Ebert
Nov 6, 2007·Journal of Cellular Biochemistry·Ji Hae JunJeong-Hwa Baek

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Citations

Mar 7, 2014·Expert Opinion on Therapeutic Targets·Angelo AvogaroGian Paolo Fadini
Nov 22, 2017·Tissue Engineering. Part a·Shin-Young ParkYang-Jo Seol
Jul 4, 2020·Molecular Medicine·Aleksandra Skubis-SikoraJoanna Gola
Sep 29, 2021·Journal of Translational Medicine·Beibei LuoLili Chen

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