High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats

Frontiers in Microbiology
Jun-Hua LiuShengyong Mao

Abstract

High-grain (HG) feeding used in intensive goat production can affect the physiology of the rumen wall, but the changes induced in the epimural bacterial community and host Toll-like receptors (TLRs) are not well understood. In this study, 10 male goats were randomly allocated to two groups and fed either a hay diet (0% grain; n = 5) or an HG diet (65% grain; n = 5). The changes in the ruminal epithelial bacterial community and expression of TLRs during long-term (7 weeks) HG feeding were determined using pyrosequencing and quantitative real-time polymerase chain reaction. Principal coordinate analysis and analysis of molecular variance (AMOVA) results showed that HG feeding caused a strong shift in bacterial composition and structure. At the genus level, our data revealed that it increased the relative abundance of taxa Butyrivibrio, unclassified Clostridiales, Mogibacterium, unclassified Anaerolineaceae, and Succiniclasticum, and decreased the proportion of unclassified Ruminococcaceae, unclassified Rikenellaceae, unclassified Erysipelotrichaceae, Howardella, and unclassified Neisseriaceae. The HG-fed goats also exhibited upregulation of the relative mRNA expression of TLR2, TLR3, and TLR5 in the rumen epithelium (P < 0.05). C...Continue Reading

References

Jan 1, 1978·Applied and Environmental Microbiology·R P McCowanJ W Costerton
Mar 1, 1988·Applied and Environmental Microbiology·M A Cotta
May 1, 1980·Applied and Environmental Microbiology·D DinsdaleR A Goodlad
May 1, 1982·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·G SemjénF Kutas
Jan 1, 1980·Applied and Environmental Microbiology·R P McCowanJ W Costerton
May 16, 2001·Science·J B Russell, J L Rychlik
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gil MelmedMaria T Abreu
Aug 21, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C Graham, N L Simmons
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sang Hoon RheeCharalabos Pothoulakis
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Sep 1, 1980·Applied and Environmental Microbiology·K J ChengJ W Costerton
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Feb 1, 2011·BMC Bioinformatics·Christopher QuincePeter J Turnbaugh
Mar 25, 2011·The Journal of Nutrition·Dulantha UlluwishewaNicole C Roy
Aug 27, 2011·Gut Microbes·Chandrashekhar CharavaryamathPhilip J Griebel
Aug 1, 2007·Animal : an International Journal of Animal Bioscience·S SadetD P Morgavi
May 2, 2012·International Journal of Dentistry·Takuichi SatoNobuhiro Takahashi
Jun 8, 2012·Science·Lora V HooperAndrew J Macpherson
Apr 16, 2013·Applied and Environmental Microbiology·R M PetriT A McAllister
Jun 7, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jun-hua LiuSheng-yong Mao
Sep 27, 2013·World Journal of Microbiology & Biotechnology·Wenjie HuoShengyong Mao

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Citations

Sep 24, 2016·Asian-Australasian Journal of Animal Sciences·Qianqian YaoZhenming Zhou
Feb 20, 2019·International Journal of Molecular Sciences·Qing NiuRuihua Huang
Jan 23, 2020·Animals : an Open Access Journal From MDPI·Guangliang XiaHongrong Wang
Jul 8, 2020·Animals : an Open Access Journal From MDPI·Dong-Hyeon KimEun-Tae Kim
Jan 30, 2019·Journal of Applied Microbiology·T O FaniyiM K Adewumi
Sep 29, 2020·Frontiers in Microbiology·Steven McLoughlinSinéad M Waters
May 8, 2019·Animal : an International Journal of Animal Bioscience·R J GruningerT A McAllister

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Datasets Mentioned

BETA
SRP052769

Methods Mentioned

BETA
electron microscopy
electrophoresis
reverse transcription PCR
scanning
transmission electron microscopy
PCR
Assay

Software Mentioned

SPSS
NCSS
MOTHUR

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