High heparin content surface-modified polyurethane discs promote rapid and stable angiogenesis in full thickness skin defects through VEGF immobilization

Journal of Biomedical Materials Research. Part a
Michelle McLuckieNicole Lindenblatt

Abstract

Three-dimensional scaffolds have the capacity to serve as an architectural framework to guide and promote tissue regeneration. Parameters such as the type of material, growth factors, and pore dimensions are therefore critical in the scaffold's success. In this study, heparin has been covalently bound to the surface of macroporous polyurethane (PU) discs via two different loading methods to determine if the amount of heparin content had an influence on the therapeutic affinity loading and release of (VEGF165 ) in full thickness skin defects. PU discs (5.4 mm diameter, 300 µm thickness, and interconnected pore size of 150 µm) were produced with either a low (2.5 mg/g) or high (6.6 mg/g) heparin content (LC and HC respectively), and were implanted into the modified dorsal skin chamber (MDSC) of C57BL/6 J mice with and without VEGF. Both low- and high-content discs with immobilized VEGF165 (LCV and HCV, respectively) presented accelerated neovascularization and tissue repair in comparison to heparin discs alone. However, the highest angiogenetic peak was on day 7 with subsequent stabilization for HCV, whereas other groups displayed a delayed peak on day 14. We therefore attribute the superior performance of HCV due to its ability ...Continue Reading

References

Jan 1, 1992·Advances in Experimental Medicine and Biology·J Folkman, Y Shing
Sep 26, 2002·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Deon BezuidenhoutPeter Zilla
Dec 25, 2002·Microscopy Research and Technique·Jie LiRobert S Kirsner
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Jul 20, 2005·Current Pharmaceutical Design·Marco RusnatiMarco Presta
Jan 3, 2006·Biochemical and Biophysical Research Communications·Barbara BrandnerAndreas J Kungl
Sep 14, 2006·Tissue Engineering·Matthias W LaschkeMichael D Menger
Nov 27, 2008·Journal of Biomaterials Applications·Deon BezuidenhoutPeter Zilla
Dec 4, 2008·Plastic and Reconstructive Surgery·Nicole LindenblattBrigitte Vollmar
Nov 13, 2009·Biomaterials·Eduardo A Silva, David J Mooney
Oct 14, 2010·Current Medicinal Chemistry·H BramfeldtP Vermette
Nov 10, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Esther WenkLorenz Meinel
Mar 30, 2012·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·M W Laschke, M D Menger
Apr 20, 2012·Vascular Cell·Reji Bhuvanendran RemaMalathi Ragunathan
Apr 14, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paola ChiodelliMarco Rusnati
Dec 18, 2015·Biotechnology Advances·Matthias W Laschke, Michael D Menger

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Citations

Oct 14, 2019·Journal of Biomedical Materials Research. Part a·Xiuzhen WanJiang Yuan
Jun 17, 2020·Chemical Society Reviews·Saeid KargozarMasoud Mozafari

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