High hydrostatic pressure affects antigenic pool in tumor cells: Implication for dendritic cell-based cancer immunotherapy

Immunology Letters
Linda UrbanovaIrena Adkins

Abstract

High hydrostatic pressure (HHP) can be used to generate dendritic cell (DC)-based active immunotherapy for prostate, lung and ovarian cancer. We showed here that HHP treatment of selected human cancer cell lines leads to a degradation of tumor antigens which depends on the magnitude of HHP applied and on the cancer cell line origin. Whereas prostate or ovarian cell lines displayed little protein antigen degradation with HHP treatment up to 300MPa after 2h, tumor antigens are hardly detected in lung cancer cell line after treatment with HHP 250MPa at the same time. On the other hand, quick reduction of tumor antigen-coding mRNA was observed at HHP 200MPa immediately after treatment in all cell lines tested. To optimize the DC-based active cellular therapy protocol for HHP-sensitive cell lines the immunogenicity of HHP-treated lung cancer cells at 150, 200 and 250MPa was compared. Lung cancer cells treated with HHP 150MPa display characteristics of immunogenic cell death, however cells are not efficiently phagocytosed by DC. Despite induction of the highest number of antigen-specific CD8+T cells, 150 MPa-treated lung cancer cells survive in high numbers. This excludes their use in DC vaccine manufacturing. HHP of 200MPa treatment...Continue Reading

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Citations

Nov 19, 2019·Frontiers in Immunology·Jitka FucikovaRadek Spisek
Aug 8, 2020·Nature Reviews. Clinical Oncology·Lorenzo GalluzziGuido Kroemer
Sep 25, 2019·Current Drug Delivery·Sepehr SoleymaniAzam Bolhassani
Apr 26, 2020·Current Opinion in Biotechnology·Silvia Martin-LluesmaLana E Kandalaft

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