Abstract
High hydrostatic pressure (HHP) can be used to generate dendritic cell (DC)-based active immunotherapy for prostate, lung and ovarian cancer. We showed here that HHP treatment of selected human cancer cell lines leads to a degradation of tumor antigens which depends on the magnitude of HHP applied and on the cancer cell line origin. Whereas prostate or ovarian cell lines displayed little protein antigen degradation with HHP treatment up to 300MPa after 2h, tumor antigens are hardly detected in lung cancer cell line after treatment with HHP 250MPa at the same time. On the other hand, quick reduction of tumor antigen-coding mRNA was observed at HHP 200MPa immediately after treatment in all cell lines tested. To optimize the DC-based active cellular therapy protocol for HHP-sensitive cell lines the immunogenicity of HHP-treated lung cancer cells at 150, 200 and 250MPa was compared. Lung cancer cells treated with HHP 150MPa display characteristics of immunogenic cell death, however cells are not efficiently phagocytosed by DC. Despite induction of the highest number of antigen-specific CD8+T cells, 150 MPa-treated lung cancer cells survive in high numbers. This excludes their use in DC vaccine manufacturing. HHP of 200MPa treatment...Continue Reading
References
Jan 1, 1986·Cancer Immunology, Immunotherapy : CII·L RichertM Shinitzky
Oct 1, 1989·International Journal of Radiation Oncology, Biology, Physics·J Y WongJ E Shively
Aug 1, 1972·British Journal of Urology·K Helmstein
Sep 15, 1995·Science·R Suto, P K Srivastava
Mar 5, 1993·Science·R I Morimoto
May 1, 1993·Cancer Immunology, Immunotherapy : CII·A EisenthalM Shinitzky
May 19, 1998·Nature Medicine·A MelcherR G Vile
May 2, 2002·Biochimica Et Biophysica Acta·Boonchai B BoonyaratanakornkitDouglas S Clark
Oct 24, 2002·Blood·Hanping FengEmmanuel Katsanis
Nov 10, 2004·Journal of Cellular Biochemistry·Mika A EloMikko J Lammi
Mar 17, 2007·Critical Reviews in Food Science and Nutrition·N K RastogiD Knorr
Oct 16, 2008·Current Medicinal Chemistry·Benjamin FreyUdo S Gaipl
Jun 6, 2009·Trends in Biotechnology·Abram AertsenChris W Michiels
Mar 9, 2010·Journal of Immunotoxicology·E M WeissB Frey
Jan 21, 2011·Cancer Metastasis Reviews·Oliver KeppGuido Kroemer
Nov 16, 2012·Nature Reviews. Cancer·Dmitri V KryskoPeter Vandenabeele
Nov 20, 2012·Annual Review of Immunology·Guido KroemerLaurence Zitvogel
Feb 7, 2014·International Journal of Cancer. Journal International Du Cancer·Jitka FucikovaRadek Spisek
May 13, 2015·Oncoimmunology·Irena AdkinsRadek Špíšek
Feb 12, 2017·PloS One·Nada HradilovaIrena Adkins
Citations
Oct 3, 2017·Tumori·Xiuying Li
Oct 14, 2017·Immunological Reviews·Benjamin FreyUdo S Gaipl
Sep 27, 2019·Frontiers in Oncology·Christoph SeitzBenjamin Frey
Nov 19, 2019·Frontiers in Immunology·Jitka FucikovaRadek Spisek
Apr 17, 2018·Reproductive Sciences·Yi-Lun WangCheng-Tao Lin
Aug 8, 2020·Nature Reviews. Clinical Oncology·Lorenzo GalluzziGuido Kroemer
Sep 25, 2019·Current Drug Delivery·Sepehr SoleymaniAzam Bolhassani
Apr 26, 2020·Current Opinion in Biotechnology·Silvia Martin-LluesmaLana E Kandalaft
Feb 13, 2021·Cancers·Michael RückertUdo S Gaipl
Jul 21, 2021·Cancer Treatment and Research Communications·Milada ZemanovaRadek Spisek