High Hydrostatic Pressure-Assisted Enzymatic Hydrolysis Affect Mealworm Allergenic Proteins.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Abir BoukilAlain Doyen

Abstract

Edible insects have garnered increased interest as alternative protein sources due to the world's growing population. However, the allergenicity of specific insect proteins is a major concern for both industry and consumers. This preliminary study investigated the capacity of high hydrostatic pressure (HHP) coupled to enzymatic hydrolysis by Alcalase® or pepsin in order to improve the in vitro digestion of mealworm proteins, specifically allergenic proteins. Pressurization was applied as pretreatment before in vitro digestion or, simultaneously, during hydrolysis. The degree of hydrolysis was compared between the different treatments and a mass spectrometry-based proteomic method was used to determine the efficiency of allergenic protein hydrolysis. Only the Alcalase® hydrolysis under pressure improved the degree of hydrolysis of mealworm proteins. Moreover, the in vitro digestion of the main allergenic proteins was increased by pressurization conditions that were specifically coupled to pepsin hydrolysis. Consequently, HHP-assisted enzymatic hydrolysis represents an alternative strategy to conventional hydrolysis for generating a large amount of peptide originating from allergenic mealworm proteins, and for lowering their immu...Continue Reading

References

Aug 24, 2000·Journal of Agricultural and Food Chemistry·T KatoT Matsuda
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Feb 4, 2010·Expert Review of Proteomics·Deborah H LundgrenDavid K Han
May 25, 2010·Journal of Food Science·Guang-Ming LiuSoheila J Maleki
Sep 3, 2013·Food Chemistry·Liya YiMartinus A J S van Boekel
Jun 23, 2015·Molecular Nutrition & Food Research·Henrike BroekmanKitty Verhoeckx
Mar 3, 2017·Journal of Agricultural and Food Chemistry·Renske H JanssenCatriona M M Lakemond
Mar 3, 2018·Biochemical and Biophysical Research Communications·Hideo OzawaYoshihiro Ochiai
May 8, 2018·Molecular Immunology·Steffie de Gier, Kitty Verhoeckx
Jul 6, 2019·Journal of Food Science and Technology·Andrea K StoneMichael T Nickerson
Feb 13, 2020·Critical Reviews in Food Science and Nutrition·Xin DongVijaya Raghavan
Apr 11, 2020·Journal of Agricultural and Food Chemistry·Chong WangLinglin Fu

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Citations

Jan 16, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexia GravelAlain Doyen
Nov 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra Dion-PoulinSylvie L Turgeon
May 1, 2021·Foods·Adrián Lomelí-MartínZamantha Escobedo-Avellaneda

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Methods Mentioned

BETA
electrophoresis
deamidation
thermal treatment

Software Mentioned

Mascot Generic
Analyst
Scaffold
Protein Pilot
Proteome
Sigma Plot
Mascot

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