High hydrostatic pressure can reverse aggregation of protein folding intermediates and facilitate acquisition of native structure

Biochemistry
B M Gorovits, P M Horowitz

Abstract

The present work demonstrates that high hydrostatic pressure can increase protein folding by reducing nonspecific aggregation. Protein aggregation is one of the main side reactions that competes with protein folding, and it typically results from interactions among partially folded intermediates. It is known that oligomeric proteins can be dissociated by the application of high hydrostatic pressure. Since protein aggregates can be described as nonspecific protein oligomers, it can be predicted that they can be completely or partially dissociated by pressure. The enzyme rhodanese is prone to slow aggregation in 3.9 M urea, and it is widely used as a model for the folding of a protein which readily aggregates. In the present study, it was demonstrated that this aggregation process could be completely reversed under high hydrostatic pressure. Release of the pressure led to renewed protein aggregation. In addition, it was demonstrated that refolding of urea-denatured rhodanese at 2 kbar pressure led to an increased yield of the native enzyme. The final recovery was increased up to approximately 25% in contrast to approximately 5% recovery observed under ambient pressure. The recovery can be further increased in the presence of 4 M ...Continue Reading

References

May 11, 1978·Nature·J H PloegmanJ Russell
Dec 15, 1992·European Journal of Biochemistry·G Zardeneta, P M Horowitz
Jan 1, 1991·Annual Review of Biochemistry·R J Ellis, S M van der Vies
Apr 11, 1991·Nature·J J Ewbank, T E Creighton
Apr 28, 1981·Biochemistry·A A Paladini, G Weber
Mar 21, 1998·Biochimica Et Biophysica Acta·B M GorovitsP M Horowitz

❮ Previous
Next ❯

Citations

Jul 9, 1999·Biotechnology and Bioengineering·D FoguelA S Robinson
Apr 7, 2004·Journal of Molecular Biology·Ralf JansenRoland Winter
Dec 19, 2003·Protein Expression and Purification·Ming LiJan-Christer Janson
Jun 11, 2005·Molecular Genetics and Metabolism·Brigitte E SchonerThomas P Burris
May 2, 2002·Biochimica Et Biophysica Acta·Wojciech DzwolakYoshihiro Taniguchi
May 2, 2002·Biochimica Et Biophysica Acta·Theodore W RandolphJohn F Carpenter
May 2, 2002·Biochimica Et Biophysica Acta·Boonchai B BoonyaratanakornkitDouglas S Clark
May 2, 2002·Biochimica Et Biophysica Acta·Jerson L SilvaDébora Foguel
Apr 5, 2001·Current Opinion in Biotechnology·E D Clark
Oct 9, 2001·Trends in Biochemical Sciences·J L SilvaC A Royer
Jul 20, 2006·The Journal of Chemical Physics·Yuichi Harano, Masahiro Kinoshita
Aug 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Débora FoguelJerson L Silva
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·R J St JohnT W Randolph
Dec 19, 2012·Archives of Biochemistry and Biophysics·Kazuyuki AkasakaYuji O Kamatari
Sep 24, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Trung Quan LuongRoland Winter
May 17, 2008·Analytical Biochemistry·Rosa Maria Chura-ChambiLigia Morganti
May 11, 2004·Protein Science : a Publication of the Protein Society·Brian G LefebvreAnne Skaja Robinson
Mar 26, 2003·Biotechnology and Bioengineering·Brian G Lefebvre, Anne Skaja Robinson
Apr 3, 2004·Biotechnology Progress·Brian G LefebvreAnne Skaja Robinson
Sep 11, 2008·Biotechnology and Bioengineering·Ryan L Crisman, Theodore W Randolph
Sep 12, 2012·Biotechnology Journal·Satoshi YamaguchiTeruyuki Nagamune
Jun 3, 2010·Biotechnology Journal·Maria K Eiberle, Alois Jungbauer
Oct 15, 2013·Biotechnology and Bioengineering·Ronald W MaurerChristopher J Roberts
Jan 18, 2005·International Journal of Pharmaceutics·Wei Wang
Jan 3, 2006·Archives of Biochemistry and Biophysics·Razieh Yazdanparast, Fariba Khodagholi
Jan 18, 2006·Biochimica Et Biophysica Acta·Filip MeersmanKarel Heremans
Jan 25, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·R Winter, W Dzwolak
Feb 12, 2009·Physical Biology·Jerson L Silva, Debora Foguel
Jan 5, 2000·The Journal of Biological Chemistry·M PandaP M Horowitz
Mar 12, 2021·Advanced Drug Delivery Reviews·Yuan ChenQi Tony Zhou
Mar 26, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe ArrabitoBruno Pignataro
Sep 25, 2009·Biochemistry·Szabolcs OsváthLászló Smeller
Jul 17, 2001·Journal of Agricultural and Food Chemistry·J YangB G Swanson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biotechnology and Bioengineering
Débora FoguelAnne Skaja Robinson
Proceedings of the National Academy of Sciences of the United States of America
Richard J St JohnTheodore W Randolph
© 2022 Meta ULC. All rights reserved