High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

BioMed Research International
Mohamed Ali Abdel-RahmanMahmoud Ali Gaber

Abstract

Optically pure lactic acid (LA) is an important chemical platform that has a wide range of industrial and biotechnological applications. Improved parameters for cost effective LA production are of great interest for industrial developments. In the present study, an alkaliphilic lactic acid bacterium, BoM 1-2, was selected among 369 newly obtained bacterial isolates. It was characterized using API 50 CHL kit and identified as Enterococcus hirae BoM 1-2 by 16S rRNA gene sequence analysis. Efficient polymer-grade L-lactic acid production was achieved at pH 9.0 and 40°C. In batch fermentation strategy using 20 g L-1 glucose, 19.6 g L-1 lactic acid was obtained with volumetric productivity of 2.18 g L-1 h-1. While using 100 g L-1 glucose, 96.0 g L-1 lactic acid was obtained with volumetric productivity of 1.07 g L-1 h-1. The highest lactic acid concentration of 180.6 g L-1 was achieved in multipulse fed batch strategy with volumetric productivity of 0.65 g L-1 h-1. To achieve higher productivity, repeated fermentation processes were applied using the two different strategies. In the first strategy, the lactic acid productivity was increased from 1.97 g L-1 h-1 to 4.48 g L-1 h-1 when the total of 10 repeated runs were carried out usi...Continue Reading

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Citations

Aug 5, 2020·Bioengineered·Guohua LiQingyang Xu
Sep 3, 2020·Biotechnology and Bioengineering·George M Teke, Robert W M Pott
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Aug 21, 2021·Critical Reviews in Biotechnology·Ernesta AugustinieneNaglis Malys

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Datasets Mentioned

BETA
KU847975
and
KU353616
MK026806

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
MEGA
SPSS
ClustalX

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