High interleukin-12 production from stimulated peripheral blood mononuclear cells of type 2 diabetes patients

Cytokine
Huang-Pin WuDuen-Yau Chuang

Abstract

Sugar control is important in patients with sepsis. Interleukin (IL)-12 induces the polarization of CD4(+) T cells to the T helper 1 (Th1) phenotype. Regulatory T (T(reg)) cells are important in immunity and disease. The aim of this work is to determine whether hyperglycemia or insulin alters IL-12 response in peripheral mononuclear cells (PBMCs). The PBMCs from 15 type 2 diabetes mellitus (DM) patients and 13 healthy controls were used for cell analysis and culture with or without treatment by glucose and insulin or stimulation by lipopolysaccharide (LPS) for 1, 2, and 3 days. The IL-12 level in the supernatant of LPS-stimulated PBMCs in the DM patients was significantly higher than that of healthy controls from day 1 to day 3. Kinetic IL-12 responses of LPS-stimulated PBMCs in the DM patients from day 1 to day 3 were significantly higher than that in healthy controls. The LPS-stimulated PBMCs under glucose treatment produced more IL-12 in DM patients but this did not happen in healthy controls. In DM patients, insulin could suppress IL-12 production from stimulated PBMCs but not with additional glucose treatment. The PBMCs of LPS-treated DM patients produced more IL-12 than that of LPS-treated healthy controls did. Hyperglyce...Continue Reading

References

Apr 1, 1997·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·M SzelachowskaI Kinalska
Jan 17, 2002·The New England Journal of Medicine·G van den BergheR Bouillon
Sep 2, 2003·Cytokine & Growth Factor Reviews·Wendy T WatfordJohn J O'Shea
Apr 20, 2004·Critical Care Medicine·R Phillip DellingerUNKNOWN Surviving Sepsis Campaign Management Guidelines Committee
Feb 19, 2005·Intensive Care Medicine·Spaska A StanilovaNoyko S Stanilov
Nov 24, 2006·Archivum Immunologiae Et Therapiae Experimentalis·Geordie RudgeIan R van Driel
Mar 22, 2007·Nature Immunology·Estelle BettelliVijay K Kuchroo
Feb 29, 2008·British Journal of Anaesthesia·J A Kolsen-PetersenE Tonnesen
Apr 10, 2008·Cytokine·Małgorzata WegnerMarzena Dworacka
Apr 24, 2009·Clinical and Vaccine Immunology : CVI·Soad NadyMohamed T Shata
Sep 3, 2009·World Journal of Gastroenterology : WJG·Hiroyuki HirasawaMasataka Nakamura
Jan 28, 2010·JAMA : the Journal of the American Medical Association·Djillali AnnaneSylvie Chevret

❮ Previous
Next ❯

Citations

Jun 29, 2012·American Journal of Reproductive Immunology : AJRI·Fariba AminzadehGholamhossein Hassanshahi
Mar 4, 2011·Cell Biochemistry and Function·Elina SkapareMaija Dambrova
Nov 12, 2013·Journal of Clinical Periodontology·Poliana M DuarteLuciana M Shaddox
Jun 4, 2015·Metabolic Syndrome and Related Disorders·Agnieszka NikołajukMarek Straczkowski
Feb 27, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Nema Ali SolimanOsama Helmy El-Khadrawy
Oct 26, 2011·Nature Reviews. Endocrinology·Hubert Kolb, Décio L Eizirik
May 11, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ana Letícia Vargas BarcelosLuiz Carlos Bodanese

❮ Previous
Next ❯

Related Concepts

Related Feeds