PMID: 9178883Jul 1, 1997Paper

High intracellular calcium levels during and after electrical discharges in molluscan peptidergic neurons

Neuroscience
K S KitsW J Wadman

Abstract

Intracellular calcium levels ([Ca2+]i) during and following electrical activity of the neuroendocrine caudodorsal cells of the pond snail (Lymnaea stagnalis) were measured in situ and in dissociated cells by combining electrical recordings and Fura-2 measurements. Caudodorsal cells are typical neuroendocrine cells that control egg laying via the release of a set of peptides during a stereotyped discharge of action potentials. Single action potentials or short trains of spikes in dissociated caudodorsal cells induced only small but consistent increases in [Ca2+]i. With longer or repeated spike trains, larger [Ca2+]i transients were measured, indicating accumulation of calcium. The calcium channel blocker Ni2+ suppressed the calcium elevation, suggesting that calcium influx occurred through voltage-activated calcium channels. Calcium levels in caudodorsal cells in situ were measured before, during and after the stereotyped firing pattern, a approximately 35-min discharge of regular spiking. Basal calcium levels in caudodorsal cells in situ were about 125 nM. During the initial phase of the discharge, the intracellular calcium level increased to approximately 250 nM. Maximal calcium levels (300-600 nM) were only reached at the fin...Continue Reading

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Citations

Jul 19, 2006·Cell and Tissue Research·Chiara SalioAdalberto Merighi
Apr 12, 2002·Progress in Neurobiology·A Merighi
Aug 27, 2010·Chemical Senses·Kathryn F Medler
Mar 10, 2011·Journal of Chemical Neuroanatomy·Adalberto MerighiLaura Lossi
Aug 15, 2014·Frontiers in Neuroinformatics·Stephan GreinGillian Queisser

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