High isolation rate and multidrug resistance tendency of penicillin-susceptible group B Streptococcus with reduced ceftibuten susceptibility in Japan

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Hirotsugu BannoYoshichika Arakawa

Abstract

Group B Streptococcus (GBS) clinical isolates with reduced penicillin susceptibility (PRGBS) have emerged through acquisition of amino acid substitutions in penicillin-binding protein 2X (PBP2X). Moreover, we also reported the emergence of penicillin-susceptible GBS clinical isolates with reduced ceftibuten susceptibility (CTBr PSGBS) due to amino acid substitutions in PBPs. However, whether or not these amino acid substitutions are responsible for the reduced ceftibuten susceptibility (RCTBS) profile remains unclear. Furthermore, the rate of CTBr PSGBS isolation and their multidrug resistance tendency remain uncertain. Therefore, we collected 377 clinical GBS isolates from multiple regions in Japan between August 2013 and August 2015. These isolates were characterized by determining MICs and sequencing the pbp2x gene. The isolation rate of CTBr PSGBS was 7.2% (27/377). CTBr PSGBS isolate harbor two types of amino acid substitutions in PBP2X [(T394A type) and (I377V, G398A, Q412L, and H438H type)]. The relevance of the amino acid substitutions found to the RCTBS was confirmed with allelic exchange techniques. Allelic exchange recombinant clones acquired two types of amino acid substitutions in PBP2X showed RCTBS. Furthermore, t...Continue Reading

References

Sep 15, 1995·Annals of Internal Medicine·L A JacksonA Schuchat
Jun 24, 1993·The New England Journal of Medicine·M M FarleyD S Stephens
Feb 19, 1999·Lancet·A Schuchat
Jan 13, 2004·Journal of Clinical Microbiology·Søren Mose HansenUffe B Skov Sørensen
Jan 31, 2004·Lancet·Paul T HeathUNKNOWN PHLS Group B Streptococcus Working Group
May 21, 2008·Antimicrobial Agents and Chemotherapy·Kouji KimuraYoshichika Arakawa
Jun 11, 2008·Antimicrobial Agents and Chemotherapy·Samira DaheshBernard W Beall
Sep 24, 2008·Antimicrobial Agents and Chemotherapy·Noriyuki NaganoYoshichika Arakawa
Apr 1, 2009·Antimicrobial Agents and Chemotherapy·Somay Yamagata MurayamaUNKNOWN Invasive Streptococcal Disease Working Group
Jun 2, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tami H SkoffStephanie J Schrag
Dec 22, 2009·The Journal of Antimicrobial Chemotherapy·Christiane GaudreauMichel Roger
Jan 3, 2012·The Journal of Antimicrobial Chemotherapy·Noriyuki NaganoYoshichika Arakawa
Jun 13, 2014·Journal of Clinical Microbiology·Noriyuki NaganoYoshichika Arakawa
Jul 15, 2015·The Journal of Antimicrobial Chemotherapy·Tomomi SekiYoshichika Arakawa
Aug 26, 2016·Journal of Clinical Microbiology·Miyuki MorozumiKimiko Ubukata
Mar 5, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B J MetcalfUNKNOWN Active Bacterial Core surveillance team

❮ Previous
Next ❯

Citations

Feb 19, 2020·Microbiology and Immunology·Yoshichika Arakawa
Oct 2, 2020·Japanese Journal of Infectious Diseases·Takumi UshinoKouji Kimura
May 5, 2020·Critical Reviews in Microbiology·Katherine HayesLesley Cotter
Nov 20, 2019·The Journal of Antimicrobial Chemotherapy·Mark van der LindenElisabete R Martins
Aug 13, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Naoya NishiyamaJiro Fujita

❮ Previous
Next ❯

Datasets Mentioned

BETA
BAA-611
NC004116

Methods Mentioned

BETA
PRGBS
electrophoresis
PSGBS

Software Mentioned

GraphPad Prism
FPQuest
GraphPad

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.