High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status

International Journal of Radiation Oncology, Biology, Physics
Akihisa TakahashiTakeo Ohnishi

Abstract

We analyzed the death pattern of human lung cancer cells harboring different p53 statuses after irradiation with different levels of linear energy transfer (LET). We used three kinds of human lung cancer cell lines with identical genotypes, except for the p53 gene. These cells were exposed to X-rays or accelerated carbon-ion beams. The cellular sensitivities were determined by a colony-forming assay. The detection and quantification of cell death (apoptosis and necrosis) were evaluated and compared by acridine orange/ethidium bromide double staining for fluorescence microscopy. We found that (1) there was no significant difference in cellular sensitivity to LET radiation >70 KeV/microm, although wild-type p53 cell sensitivity to X-rays was higher than that of mutated p53 or p53-null cells; (2) low-LET radiation effectively induced apoptosis in wild-type p53 cells as compared with mutated p53 and p53-null cells; and (3) high-LET radiation induced p53-independent apoptosis. Our findings suggest that high-LET radiotherapy is expected to be a valid application for patients carrying mutated p53 cancer cells. We proposed that the elucidation of the p53-independent apoptosis-related genes might provide new insights into radiotherapy f...Continue Reading

References

May 8, 1992·Science·S E KernB Vogelstein
Jul 2, 1992·Nature·D P Lane
Dec 1, 1983·Molecular and Cellular Biology·N C ReichA J Levine
Dec 1, 1982·International Journal of Radiation Oncology, Biology, Physics·C A TobiasT C Yang
Mar 1, 1995·Radiation and Environmental Biophysics·J H HendryA Merritt
Jul 1, 1994·The Journal of General Virology·A C Vasconcelos, K M Lam
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M Lee, A Bernstein
Nov 1, 1995·Current Opinion in Oncology·S W Lowe
May 10, 2000·International Journal of Radiation Oncology, Biology, Physics·A TakahashiT Ohnishi
Jun 24, 2000·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·M LeiteJ E Guimarães
Oct 30, 2001·International Journal of Radiation Biology·A TakahashiT Ohnishi
Aug 17, 2002·Canadian Journal of Physiology and Pharmacology·D CoelhoP Bischoff

❮ Previous
Next ❯

Citations

May 8, 2010·Applied Biochemistry and Biotechnology·Satomi Mizukami-MurataMasaharu Hoshi
Jan 18, 2006·Journal of Biomaterials Science. Polymer Edition·F BianchiA Ahluwalia
Apr 25, 2012·Radiation Oncology·Katsutoshi MiuraMasato Hareyama
Jul 29, 2009·International Journal of Radiation Biology·Wael S Al-JahdariTakashi Nakano
Sep 4, 2009·International Journal of Radiation Biology·Koichi Ando, Yuki Kase
Feb 24, 2016·Frontiers in Oncology·Kathryn D HeldAkihisa Takahashi
Feb 12, 2009·International Journal of Radiation Biology·M S Sasaki
Nov 22, 2005·International Journal of Radiation Biology·A TakahashiT Ohnishi
Feb 27, 2008·International Journal of Radiation Biology·Xin DuanZheng-Hua Wu
Feb 27, 2008·International Journal of Radiation Biology·Akitoshi KamidaYoshiaki Yura
Apr 16, 2008·International Journal of Radiation Oncology, Biology, Physics·Akihisa TakahashiTakeo Ohnishi
Apr 4, 2007·Annals of the New York Academy of Sciences·Aleksandra M Ristic-FiraGiacomo Cuttone
Apr 6, 2016·Frontiers in Oncology·Christine E HellwegChrista Baumstark-Khan
Jun 24, 2010·International Journal of Radiation Biology·Thomas E SchmidBarbara Röper
Jan 11, 2007·The Journal of Veterinary Medical Science·Takehiko KakizakiNobuhiko Ito
Jul 1, 2006·Radiation Research·Nobuyuki HamadaYasuhiko Kobayashi
Feb 9, 2017·Canadian Journal of Physiology and Pharmacology·Lorena Dos Santos BubniakMaria Cláudia Santos-Silva
Oct 31, 2007·International Journal of Radiation Oncology, Biology, Physics·Maria Alejandra DagrosaMario Alberto Pisarev
Oct 5, 2017·Physics in Medicine and Biology·Christian P Karger, Peter Peschke
Aug 1, 2018·International Journal of Molecular Sciences·Christine E HellwegChrista Baumstark-Khan
Oct 14, 2008·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Hae-June LeeSung-Ho Kim
Apr 29, 2005·Uchū Seibutsu Kagaku·Tetsuya KawataFrancis A Cucinotta
Jan 18, 2006·In Vitro Cellular & Developmental Biology. Animal·Feng-Ling MinGuang-Ming Zhou
Sep 13, 2012·Experimental and Therapeutic Medicine·Ichiro OtaTakeo Ohnishi
Jul 9, 2020·International Journal of Molecular Sciences·Camille HuartAnne-Catherine Wéra
Oct 8, 2008·Journal of Radiation Research·Nobuyuki Hamada
Aug 4, 2010·Journal of Radiation Research·Nobuyuki HamadaTeruki Teshima
Jul 1, 2018·International Journal of Particle Therapy·Christine E HellwegChrista Baumstark-Khan
Jul 1, 2018·International Journal of Particle Therapy·Stephanie K SchaubUpendra Parvathaneni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis