High LET radiation shows no major cellular and functional effects on primary cardiomyocytes in vitro

Life Sciences in Space Research
Anja HeselichChristiane Thielemann

Abstract

It is well known that ionizing radiation causes adverse effects on various mammalian tissues. However, there is little information on the biological effects of heavy ion radiation on the heart. In order to fill this gap, we systematically examined DNA-damage induction and repair, as well as proliferation and apoptosis in avian cardiomyocyte cultures irradiated with heavy ions such as titanium and iron, relevant for manned space-flight, and carbon ions, as used for radiotherapy. Further, and to our knowledge for the first time, we analyzed the effect of heavy ion radiation on the electrophysiology of primary cardiomyocytes derived from chicken embryos using the non-invasive microelectrode array (MEA) technology. As electrophysiological endpoints beat rate and field action potential duration were analyzed. The cultures clearly exhibited the capacity to repair induced DNA damage almost completely within 24 h, even at doses of 7 Gy, and almost completely recovered from radiation-induced changes in proliferative behavior. Interestingly, no significant effects on apoptosis could be detected. Especially the functionality of primary cardiac cells exhibited a surprisingly high robustness against heavy ion radiation, even at doses of up ...Continue Reading

Citations

Apr 2, 2020·Nature Reviews. Cardiology·Joerg Herrmann
Nov 18, 2020·Biomedicines·Dragoș-Mihai RomitanTudor-Eliade Ciuleanu
Apr 28, 2021·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Catherine M DavisDawn E Bowles

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