Abstract
Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 +/- 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 +/- 2.1), rabbit (4.0 +/- 1.1), and guinea pig (0.3 +/- 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively.
References
Mar 1, 1978·European Journal of Biochemistry·A G Hovanessian, I M Kerr
Nov 1, 1992·Clinical Immunology and Immunopathology·M E MieleN C Kan
Apr 1, 1992·Acta Paediatrica·H I HuppertzH W Kreth
Jan 1, 1986·Journal of Biochemistry·J Sokawa, Y Sokawa
Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ShindoY Sokawa
Sep 1, 1986·Journal of Clinical Microbiology·H SuginoY Sokawa
Oct 1, 1985·Journal of Biochemistry·H SawaiS Watanabe
Oct 1, 1974·The Journal of General Virology·J L RiggsE H Lennette
Jun 1, 1983·The Journal of Experimental Medicine·O T PrebleM I Johnston
Feb 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·C Buffet-Janvresse, A G Hovanessian
Jul 1, 1984·Journal of Biochemistry·J SokawaY Sokawa
Jan 1, 1993·Advances in Virus Research·G C Sen, R M Ransohoff
Jun 1, 1994·Journal of Interferon Research·Y SokawaK Takeda
May 1, 1994·The Journal of Infectious Diseases·S RusconiM Galli
Oct 1, 1993·Liver·A SolinasM S Diana
Dec 1, 1995·British Journal of Cancer·B C Millar, J B Bell
Oct 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M Asada-KubotaY Sokawa
Jan 1, 1996·The Journal of Veterinary Medical Science·A IwataS Ueda
Oct 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P SalmonA Darragh
Mar 1, 1997·Anatomy and Embryology·M Asada-KubotaY Sokawa
Feb 25, 1998·Biochimica Et Biophysica Acta·A YamamotoY Sokawa
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
May 20, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·D Rebouillat, A G Hovanessian
Mar 7, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R J MotzerL Hooftman
Sep 12, 2002·Cellular and Molecular Life Sciences : CMLS·S EskildsenJ Justesen
Oct 25, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Shigeru KakutaYoichiro Iwakura