Sep 18, 2009

High-level chromate resistance in Arthrobacter sp. strain FB24 requires previously uncharacterized accessory genes

BMC Microbiology
Kristene L HenneAllan Konopka

Abstract

The genome of Arthrobacter sp. strain FB24 contains a chromate resistance determinant (CRD), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. The CRD includes chrA, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of ChrB, a putative regulatory protein. There are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode an oxidoreductase (most similar to malate:quinone oxidoreductase), a functionally unknown protein with a WD40 repeat domain and a lipoprotein. To delineate the contribution of the CRD genes to the FB24 chromate [Cr(VI)] response, we evaluated the growth of mutant strains bearing regions of the CRD and transcript expression levels in response to Cr(VI) challenge. A chromate-sensitive mutant (strain D11) was generated by curing FB24 of its 96-kb plasmid. Elemental analysis indicated that chromate-exposed cells of strain D11 accumulated three times more chromium than strain FB24. Introduction of the CRD into strain D11 conferred chromate resistance comparable to wild-type levels, whereas deletion of specific regions of the CRD led to decreas...Continue Reading

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Chromium product (pharmacologic preparation)
Gene Expression Regulation, Bacterial
Biological Adaptation to Stress
ATP8A2 gene
Chromium
Bacterial Proteins
Hydrogen Peroxide
Pseudomonas aeruginosa (antigen)
Genome

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