Abstract
Rituximab, a chimeric anti-CD20 monoclonal antibody, is one of the most successful biomedicines and has been used to treat at least 370,000 patients with indolent, aggressive non-Hodgkin's lymphoma and other malignant diseases. However, the global demand for rituximab and other therapeutic monoclonal antibodies is exponentially increasing and barely able to be met by current manufacturing capacities of mammalian cell culture. The mammary gland bioreactor has been regarded as an ideal substitute for mammalian cell culture to mass-produce recombinant monoclonal antibodies at the lowest possible cost. Here, we show a feasible model to produce recombinant anti-CD20 antibodies in the mammary glands of transgenic animals. Six lines of transgenic mice were generated by co-microinjection of the two expression cassettes that can specially express the chimeric light and heavy chain of anti-CD20 mAbs in the milk of transgenic animals. The recombinant antibodies were detected in the milk of transgenic mice with the highest expression level up to 17 microg/mul and could specifically bind the CD20 surface antigens on human B-lymphoma cells.
References
Mar 23, 1989·Nature·D TalbotD R Greaves
Nov 1, 1980·Cell·P A HieterP Leder
Jun 1, 1982·Cell·N TakahashiT Honjo
Aug 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·J LimontaJ de la Fuente
Apr 29, 1998·Nature Biotechnology·J CastillaL Enjuanes
Apr 29, 1998·Journal of Virology·I SolaL Enjuanes
Dec 3, 1998·Research in Immunology·M W YoungM Harvey
Jan 29, 2000·Journal of Immunological Methods·D P PollockH M Meade
Aug 22, 2000·Transgenic Research·P van Kuik-RomeijnH A de Boer
Sep 28, 2000·Cellular Immunology·D FliegerI Schmidt-Wolf
Feb 27, 2001·Journal of Virology·A F KolbS G Siddell
Sep 5, 2002·International Journal of Health Services : Planning, Administration, Evaluation·Eeva Ollila, Meri Koivusalo
Dec 17, 2002·Current Opinion in Biotechnology·Louis Marie Houdebine
Mar 22, 2003·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J BoyeA Engert
Oct 11, 2003·Blood·Mark S Cragg, Martin J Glennie
May 22, 2004·Nature Reviews. Drug Discovery·Janice Reichert, Alex Pavolu
Mar 23, 2005·Critical Reviews in Oncology/hematology·M Stern, R Herrmann
Jun 18, 2005·Arthritis Research & Therapy·Ann Mohrbacher
Sep 10, 2005·Nature Biotechnology·Janice M ReichertMatthew C Dewitz
Sep 10, 2005·Nature Biotechnology·Gregory P Adams, Louis M Weiner
Mar 31, 2006·Journal of Virology·Shuyang YuNing Li
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M D Pescovitz
Aug 11, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Suzanne S Farid
Citations
Jan 9, 2009·Transgenic Research·Ran ZhangNing Li
Jun 22, 2010·Transgenic Research·Zuyong HeYaosheng Chen
Dec 25, 2015·Bioconjugate Chemistry·Zhan ZhouZhiguo Su
Apr 25, 2012·Science China. Life Sciences·Ping WangYi Sun
Dec 15, 2010·Biochimie·Veronika YuskevichSergey Deyev
Aug 19, 2020·Preparative Biochemistry & Biotechnology·Guihua GongYoujia Hu
Sep 6, 2018·Scientific Reports·Ran ZhangNing Li
Dec 29, 2009·Médecine sciences : M/S·Stéphane Olivier, Majid Mehtali