PMID: 11334980May 4, 2001Paper

High level expression of a human rabies virus-neutralizing monoclonal antibody by a rhabdovirus-based vector

Journal of Immunological Methods
K MorimotoB Dietzschold

Abstract

Humans exposed to rabies virus must be promptly treated by passive immunization with anti-rabies antibody and active immunization with rabies vaccine. Currently, antibody prepared from pooled human serum or from immunized horses is utilized. However, neither of these reagents are readily available, entirely safe, or consistent in their biological activity. An ideal reagent would consist of a panel of human monoclonal antibodies. Such antibodies are now available, their only drawback being the cost of production. Using recombinant technology, we constructed a rabies virus-based vector which expresses high levels (approximately 60 pg/cell) of rabies virus-neutralizing human monoclonal antibody. The vector is a modified vaccine strain of rabies virus in which the rabies virus glycoprotein has been replaced with a chimeric vesicular stomatitis virus glycoprotein, and both heavy and light chain genes encoding a human monoclonal antibody have been inserted. This recombinant virus can infect a variety of mammalian cell lines and is non-cytolytic, allowing the use of cell culture technology routinely employed to produce rabies vaccines.

References

Nov 1, 1988·Reviews of Infectious Diseases·J H Steele
Feb 1, 1989·Vaccine·H WildeS Chutivongse
Feb 10, 1994·Journal of Immunological Methods·R J Owens, R J Young
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·K MorimotoB Dietzschold

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Citations

Jul 30, 2002·The Lancet Infectious Diseases·Charles E RupprechtThiravat Hemachudha
Dec 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bishnu P DeJulie L Boyer
Aug 31, 2004·Journal of Clinical Pathology·K A SmithJ Greenman
Nov 6, 2009·PLoS Neglected Tropical Diseases·Thomas MüllerMarie Paule Kieny
Sep 24, 2011·Human Vaccines·Anurag SharmaStefan Worgall
Jul 6, 2006·Virology·Mary Ellen SmithMatthias J Schnell
Jan 26, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuhiko KasuyaRonald G Crystal
Aug 9, 2003·Expert Review of Vaccines·Bernhard DietzscholdMatthias J Schnell

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