High level expression of C-terminal truncated recombinant chicken interferon-gamma in baculovirus vector system

The Journal of Veterinary Medical Science
Kazuaki TakeharaMasayuki Nakamura

Abstract

Chicken interferon-gamma (ChIFN-gamma) was expressed by baculovirus in a C-terminal truncated form, namely ChIFN-gammaT, to accelerate the secretion of the expressed protein. It is also expressed as ChIFN-gammaT bearing poly His tag, ChIFN-gammaTHis, for easy purification. The expressed proteins were detected by SDS-PAGE analysis with Coomassie brilliant blue staining. The purified ChIFN-gammaTHis with nickel chelated column showed anti-viral activity in vitro and stimulation of the secretion of nitrogen intermediates such as nitric oxide in chicken peripheral blood mononuclear cells. Antiserum against ChIFN-gammaTHis recognized the 15 kDa, 16 kDa, and 32 kDa bands that seemed to be an unglycosylated monomer, a glycosylated monomer, and a homodimer of ChIFN-gammaTHis in the culture supernatant, respectively. The anti-serum also recognized around 14 kDa and 28 kDa bands in the sera of chickens or concanavalin A stimulated spleen cell culture supernatants that seemed to be monomeric and dimeric forms of a natural ChIFN-gamma, respectively.

References

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Oct 1, 1996·European Journal of Immunology·K C WeiningP Staeheli
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Citations

Jul 9, 2011·Bioscience, Biotechnology, and Biochemistry·Wen-Shuo ChenTzong-Yuan Wu
Feb 5, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Amirul I MallickShayan Sharif
Jan 8, 2004·The Journal of Veterinary Medical Science·Kazuaki TakeharaMasayuki Nakamura
Feb 9, 2005·The Journal of Veterinary Medical Science·Ruttapong RuttanapummaKazuaki Takehara
Oct 28, 2016·GM Crops & Food
Feb 25, 2015·Prikladnaia biokhimiia i mikrobiologiia·M A TsygankovM V Padkina
Aug 17, 2021·Biotechnology and Bioprocess Engineering : BBE·Siddhesh B GhagVishwas A Bapat

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