PMID: 2497580May 1, 1989Paper

High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors

Virology
V A Luckow, M D Summers

Abstract

High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot blot analysis of total cytoplasmic RNA. When the polyhedrin ATG start codon was converted to ATT by site-directed mutagenesis, translation initiated at downstream ATG codons resulting in high yields of nonfused foreign proteins. When a stop codon was inserted downstream from and in phase with the polyhedrin ATG codon but upstream from the ATG of a foreign gene, nonfused proteins were also produced, but at lower levels. The level of steady-state polyhedrin gene-promoted mRNA was not affected by the mutation from ATG to ATT or the insertion of in phase stop codons downstream from the polyhedrin ATG.

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Citations

Jul 5, 1997·Biotechnology and Bioengineering·M L KennardW A Jefferies
Mar 15, 1992·Biotechnology and Bioengineering·D A Lindsay, M J Betenbaugh
Jan 5, 1993·Biotechnology and Bioengineering·S C WuJ C Liao
Mar 1, 1996·Protein Science : a Publication of the Protein Society·M GuoK E Neet
Apr 1, 1994·In Vitro Cellular & Developmental Biology. Animal·J E MaruniakJ J Rodrigues
May 1, 1993·In Vitro Cellular & Developmental Biology. Animal·T R DavisH A Wood
Nov 1, 1990·Molecular Immunology·E BaixerasF Triebel
Jan 1, 1995·Journal of Virological Methods·B C Bonning, B D Hammock
Apr 1, 1994·Molecular and Biochemical Parasitology·S LongacreP H David
Jun 25, 1993·Biochimica Et Biophysica Acta·B HerrenM Pech
Nov 1, 1992·Virus Research·K A MatherJ L McKimm-Breschkin
Jan 1, 1995·Biochimie·M MassonC Niedergang
Sep 1, 1995·Veterinary Microbiology·J M WhalleyH J Field
Jun 15, 1994·European Journal of Pharmacology·E M ParkerG T Gaughan
Jun 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·T BarkhemS Nilsson
Oct 6, 1998·Journal of Immunological Methods·R VermaA J George
Sep 5, 1998·Biochimica Et Biophysica Acta·W K HoA O Wong
Sep 4, 1999·FEMS Microbiology Letters·G de KortJ A van de Klundert

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