High-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium

Plant Molecular Biology
T HattoriK Nakamura

Abstract

Sporamin, the tuberous root storage protein of the sweet potato, accounts for about 60 to 80% of the total soluble protein of this organ. The amount of sporamin present in other organs is very low, or even not detectable, in the normal field-grown plants. However, the stem of sweet potato plantlets grown axenically on agar medium containing sucrose was found to accumulate large amounts of sporamin. Two-dimensional gel electrophoretic profiles of sporamin precursors synthesized in vitro by poly(A)+ RNA are indistinguishable between tuberous roots of the field-grown plants and stems of the axenically cultured plants, suggesting that an essentially identical set of the members of sporamin multigene family are expressed in these two organs under different growth conditions. Transgenic tobacco plants having a CAT (chloramphenicol acetyltransferase) fusion gene with the 5' upstream region of a sporamin A gene, gSPO-A1, show preferential expression of CAT activity in stems when the plants are maintained in axenic culture on sucrose medium as is the case for sporamin in sweet potato. Deletion analysis revealed that the DNA sequence of gSPO-A1 between -94 and -305, relative to the transcription start site, is important for its expressio...Continue Reading

References

Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M FrommV Walbot

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Citations

Oct 21, 1992·Gene·N YoshidaK Nakamura
Jan 17, 2003·Plant Physiology·Rossitza AtanassovaSerge Delrot
Jan 15, 2002·Plant Physiology·Gustavo Bonaventure, John B Ohlrogge
Sep 13, 2003·Plant Physiology·Rüdiger Hauschild, Antje von Schaewen
Mar 9, 1999·Plant Physiology·J V PegoS C Smeekens
Aug 1, 1995·Biological Reviews of the Cambridge Philosophical Society·P R Shewry
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·K. E. Koch
Feb 11, 2005·Journal of Plant Physiology·Masaru TanakaMakoto Nakatani
Sep 1, 2006·Plant Science : an International Journal of Experimental Plant Biology·Huai-Ju ChenKai-Wun Yeh
Feb 7, 2012·Biotechnology Advances·Rajendran Senthilkumar, Kai-Wun Yeh
Aug 12, 2000·Journal of Experimental Botany·J V PegoS C Smeekens
Dec 1, 1996·European Journal of Epidemiology·E E Udo, W B Grubb
May 24, 2019·Journal of Proteome Research·Thualfeqar Al-MohannaSorina C Popescu

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