PMID: 9178561May 1, 1997Paper

High level expression of XMP aminase in Escherichia coli and its application for the industrial production of 5'-guanylic acid

Bioscience, Biotechnology, and Biochemistry
Tatsuro FujioA Maruyama

Abstract

To improve the efficiency of the enzymatic conversion of 5'-xanthylic acid (XMP) to 5'-guanylic acid (GMP), we attempted to increase the activity of the conversion enzyme, XMP aminase (GMP synthetase) encoded by the guaA gene in Escherichia coli. By connecting the PL promoter of lambda phage, the SD sequence of trpL of E. coli, and ATG, at a suitable position upstream of the guaA gene, we obtained plasmid pPLA66. Sequencing of the nucleotides of the upstream region of the guaA gene on pPLA66 showed that the C-terminal region of the guaB gene, which encodes IMP dehydrogenase, was conserved and a short peptide consisted of 14 amino acids was coded. E. coli MP347/pPLA66 showed an increase in the activity of approximately 370 times when compared with that of the strain MM294, and the amount of the enzyme protein represented approx. 34% of the total cellular protein. Strain MP347/pPLA66 was cultivated in a 5-liter jar fermentor using a medium which contained mainly corn steep liquor. The culture broth had high XMP aminase activity. In the conversion reaction using mixed broths consisted of 600 ml of XMP-fermentation broth of Corynebacterium ummoniagenes KY13203 and 30 ml of cultured broth of E. coli MP347/pPLA66, a surfactant, Nymee...Continue Reading

Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Jeong Hyun KimBaek Rak Lee
Dec 22, 1999·Current Opinion in Biotechnology·S i Hashimoto, A Ozaki
Oct 20, 2005·Journal of Bioscience and Bioengineering·A KamedaT Noguchi
Sep 19, 2015·Critical Reviews in Biotechnology·Carrie PerkinsArnold L Demain
Nov 26, 2008·Biotechnology Advances·Jingwen ZhouJian Chen
Aug 23, 2008·Metabolic Engineering·Kiyotaka Y HaraHideo Mori
May 9, 2015·Applied Microbiology and Biotechnology·Jesús Fernández-Lucas
Jan 11, 2014·Bioengineered·Stephanie KlatteVolker F Wendisch
Jun 26, 2009·FEMS Microbiology Letters·Kiyotaka Y HaraHideo Mori
May 2, 2001·Bioscience, Biotechnology, and Biochemistry·H MatsuiO Kurahashi
Feb 24, 2006·Journal of Biomolecular Screening·Kiyotaka Y Hara, Hideo Mori

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