High-level expression, purification and properties of an Endochitinase gene without signal peptide from Lecanicillium lecanii 43H in Pichia pastoris

Molecular Biology Reports
Huu Quan NguyenHoang Mau Chu

Abstract

Chitinases play the key role in hydrolysis of chitin, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. The Lecanicillium lecanii strain 43H was used as the source for the Endochitinase gene without signal peptide (mchit1). This mchit1 gene was cloned and sequenced. The recombinant Endochitinase non signal peptide was overexpressed in Pichia pastoris X33 with a level of 2.048 U mL-1 culture supernatant. The molecular mass of the purified recombinant Endochitinase (rmchit1) without signal peptide was 43 kDa. Metal ions, detergents, and organic solvents tested indicated a significantly influence on rmchit1 activity. The obtained results demonstrated that signal peptides affect the yield expression, purification methods, recovery as well as the physicochemical properties of the enzyme.

References

Dec 12, 2002·Chemical Reviews·Mark PaetzelRoss E Dalbey
Aug 9, 2006·Protein Expression and Purification·Dinh Thi QuyenSy Le Thanh Nguyen

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BETA
PCR
electrophoresis

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DNAStar

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