High Level Extracellular Secretion of Organophosphorous Hydrolase of Flavobacterium sp. in Escherichia coli BL21(DE3)pLysS

Biotechnology and Applied Biochemistry
S K Falahati-PourFereshteh Haghighi

Abstract

Organophosphorus (OPs) compounds are widely used in many pesticides, insecticides and chemical nerve agents. These compounds are hazardous for humans and environment. There are many reports on detoxification of these compounds, among them enzymatic cleavage of these compounds with Organophosphorus hydrolase (OPH) has been taken into more consideration. Several studies have been performed to improve OPH secretion in E. coli by different signal peptides which have not been successful. In this study, to achieve the extracellular secretion of OPH in E. coli, the complete opd gene along with its native signal peptide was codon optimized and expressed in E. coli BL21(DE3)pLysS. The Culture medium showed OPH activity after 2, 4 and 6 h of induction time. The extracellular Secretion of OPH was also confirmed by SDS-PAGE and western blot analysis. The effects of different factors in growth medium were also investigated regarding expression and extra cellular secretion of OPH. It appears that the secretion of OPH into the extracellular medium is highly affected by culture conditions. Therefore our results revealed that the recombinant OPH was successfully secreted into the extracellular medium. This secretion system can be considered as ...Continue Reading

References

Nov 1, 1996·Molecular Microbiology·B C Berks
Jul 27, 1999·Chemico-biological Interactions·B D Di SioudiJ R Wild
Aug 19, 2000·Nature·J C SamuelsonR E Dalbey
Jun 29, 2002·Applied and Environmental Microbiology·Irene HorneJohn G Oakeshott
Feb 18, 2004·Applied Microbiology and Biotechnology·J H Choi, S Y Lee
Jan 21, 2006·Protein Engineering, Design & Selection : PEDS·Catherine Mee-Hie ChoWilfred Chen
Aug 7, 2007·Biochemistry·Sharyn L Rusch, Debra A Kendall
Jul 15, 2009·Biotechnology Letters·Ye Ni, Rachel Chen
Oct 5, 2010·Applied Microbiology and Biotechnology·Casey M Theriot, Amy M Grunden
May 14, 2011·Biotechnology Journal·Yaramah M ZaluckiMichael P Jennings
Aug 11, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Katsumi TakayamaMitsuyoshi Ueda
Jun 12, 2012·Nature Reviews. Microbiology·Tracy Palmer, Ben C Berks
Mar 27, 2013·Applied Microbiology and Biotechnology·Kheng Oon LowRosli Md Illias
Sep 17, 2013·Biotechnology and Applied Biochemistry·Garshasb RigiKambiz Akbari Noghabi

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