High-level gene expression in Lactobacillus plantarum using a pheromone-regulated bacteriocin promoter

Letters in Applied Microbiology
Geir MathiesenVincent G H Eijsink

Abstract

To use promoters and regulatory genes involved in the production of the bacteriocin sakacin P to obtain high-level regulated gene expression in Lactobacillus plantarum. In a plasmid containing all three operons naturally involved in sakacin P production, the genes encoding sakacin P and its immunity protein were replaced by the aminopeptidase N gene from Lactococcus lactis (pepN) or the beta-glucuronidase gene from Escherichia coli (gusA). The new genes were precisely fused to the start codon of the sakacin P gene and the stop codon of the immunity gene. This set-up permitted regulated (external pheromone controlled) overexpression of both reporter genes in L. plantarum NC8. For PepN, production levels amounted to as much as 40% of total cellular protein. Promoters and regulatory genes involved in production of sakacin P are suitable for establishing inducible high-level gene expression in L. plantarum. This study describes a system for controllable gene expression in lactobacilli, giving some of the highest expression levels reported so far in this genus.

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Citations

Aug 26, 2006·Archives of Microbiology·Trinelise BlomqvistLeiv Sigve Håvarstein
Aug 10, 2005·Applied Microbiology and Biotechnology·Igor Mierau, Michiel Kleerebezem
Jun 21, 2012·Applied Microbiology and Biotechnology·N BöhmerL Fischer
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Nov 15, 2011·Journal of Bacteriology·Lasse FredriksenWolfgang Egge-Jacobsen
Nov 21, 2014·Microbial Cell Factories·Christopher TauerReingard Grabherr
Nov 1, 2011·Protein Expression and Purification·Hoang Anh NguyenDietmar Haltrich
Feb 27, 2008·Journal of Applied Microbiology·G MathiesenV G H Eijsink
Aug 23, 2011·Biotechnology Journal·Clemens PeterbauerDietmar Haltrich
Mar 8, 2011·Microbial Biotechnology·Tri DuongTodd R Klaenhammer
Mar 13, 2018·Annual Review of Biomedical Engineering·Josef R BoberNikhil U Nair
Sep 6, 2015·Applied and Environmental Microbiology·Caitriona M GuinanePaul D Cotter
Apr 27, 2019·Microbial Cell Factories·Hoang-Minh NguyenThu-Ha Nguyen

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