High Level of APOA1 in Blood and Maternal Fetal Interface Is Associated With Early Miscarriage

Reproductive Sciences
Priyanka VermaKiran Singh

Abstract

Early miscarriage (EM) is one of the most devastating obstetrical complications globally affecting the quality of women's life. In the present study, we aimed to identify proteins that correlate with and could act as biomarkers for EM. We performed 2-dimensional gel electrophoresis in chorionic villi samples followed by mass spectrometry for identification of differential protein expression with EM. Proteomic studies detected a total 124 protein spots, out of which 83 spots were differentially expressed between EM and controls in chorionic villi samples. Matrix assisted laser desorbtion/ionization-time of flight (MALDI-TOF) mass spectrometry analysis revealed Apolipoprotein A1 (APOA1) to be the most upregulated protein in the EM group that was validated by Western blotting and Enzyme-linked immunosorbent assay (ELISA) . We found low but not statistically significant level of APOA1 on 21st day of menstruation in comparison to the 7th day. APOA1 level was observed to be the lowest in the first trimester. Hence, this study suggests that low APOA1 expression is critical in establishing pregnancy and elevated APOA1 expression in chorionic villi correlates with EM. Similar observation in serum samples suggests its potential as a mark...Continue Reading

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Citations

Aug 29, 2020·Biology of Reproduction·Xi GuoXiaoyan Chen
Mar 7, 2021·BMJ Open Diabetes Research & Care·Manjunath RamanjaneyaAbdul Badi Abou-Samra
Mar 3, 2021·Wellcome Open Research·Maria C MagnusAbigail Fraser
Jul 3, 2021·International Journal of Molecular Sciences·Dorota PierzchałaMariusz Pierzchała

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