High level of chromosome 15 aneuploidy in head and neck squamous cell carcinoma lesions identified by FISH analysis: limited value of beta2-microglobulin LOH analysis

Tissue Antigens
Geert J P A KoeneMarcel Tilanus

Abstract

In cancer research, loss of heterozygosity (LOH), defined by microsatellite markers, is frequently used in the identification of gene loss. Especially, genomic alterations in the human leukocyte antigen (HLA) genes and the beta2-microglobulin (beta2m) gene on chromosome 15 are of interest regarding their function in the immune system. Because LOH analysis detects any allelic imbalance and not just allelic loss, we evaluated the LOH analysis in 11 head and neck squamous cell carcinoma (HNSCC) lesions using fluorescence in situ hybridization (FISH). The 11 tumors were selected out of 53 HNSCC lesions based upon beta2m LOH analysis and beta2m expression. Centromere 1 and 15 FISH were developed to determine the chromosome 15 copy number. Sequence-based mutation analysis of beta2m was conducted on tumors without beta2m expression; no mutations in the coding sequences were found. For five HNSCC lesions with LOH and beta2m expression, centromere 15 FISH indicated gain rather than loss. In the majority of the 11 HNSCC lesions, FISH showed centromere 1 and 15 heterogeneity throughout the tumor. Moreover, FISH indicated a more complex chromosome 1 and 15 distribution than could be concluded from microsatellite LOH analysis. Our results s...Continue Reading

References

Oct 1, 1993·Immunology Today·F GarridoP L Stern
Jan 1, 1995·Advances in Cancer Research·F GarridoF Ruiz-Cabello
Mar 1, 1996·The Journal of Experimental Medicine·T Boon, P van der Bruggen
Mar 4, 1998·Head & Neck·U BockmühlI Petersen
Jan 13, 2000·Immunological Reviews·J C Solheim
Jun 27, 2000·Advances in Oto-rhino-laryngology·J AkervallF Mertens
Jul 8, 2000·Tissue Antigens·L M RamalF Garrido
Sep 7, 2000·The New England Journal of Medicine·J Klein, A Sato
Sep 14, 2000·The New England Journal of Medicine·J Klein, A Sato
Dec 18, 2001·Genes, Chromosomes & Cancer·Ulrike BockmühlIver Petersen
Dec 4, 2002·Virchows Archiv : an International Journal of Pathology·Ulrike Bockmühl, Iver Petersen
Dec 10, 2002·International Journal of Cancer. Journal International Du Cancer·Ekaterina S JordanovaPhilip M Kluin
Dec 13, 2002·Vaccine·Michael CampoliSoldano Ferrone
May 8, 2003·Cell Cycle·Peter Duesberg, Ruhong Li

❮ Previous
Next ❯

Citations

Oct 16, 2007·Cancer Immunology, Immunotherapy : CII·Rosa MéndezFederico Garrido
Oct 20, 2010·International Journal of Cancer. Journal International Du Cancer·Rafael CarreteroFederico Garrido
Aug 15, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Micaela Poetsch, Britta Kleist

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.