High level of coreceptor-independent HIV transfer induced by contacts between primary CD4 T cells.

The Journal of Biological Chemistry
Julià BlancoJosé A Esté

Abstract

Cell-to-cell virus transmission is one of the most efficient mechanisms of human immunodeficiency virus (HIV) spread, requires CD4 and coreceptor expression in target cells, and may also lead to syncytium formation and cell death. Here, we show that in addition to this classical coreceptor-mediated transmission, the contact between HIV-producing cells and primary CD4 T cells lacking the appropriate coreceptor induced the uptake of HIV particles by target cells in the absence of membrane fusion or productive HIV replication. HIV uptake by CD4 T cells required cellular contacts mediated by the binding of gp120 to CD4 and intact actin cytoskeleton. HIV antigens taken up by CD4 T cells were rapidly endocytosed to trypsin-resistant compartments inducing a partial disappearance of CD4 molecules from the cell surface. Once the cellular contact was stopped, captured HIV were released as infectious particles. Electron microscopy revealed that HIV particles attached to the surface of target cells and accumulated in large (0.5-1.0 microm) intracellular vesicles containing 1-14 virions, without any evidence for massive clathrin-mediated HIV endocytosis. The capture of HIV particles into trypsin-resistant compartments required the availabil...Continue Reading

References

May 1, 1994·Journal of Virology·R Pearce-PrattD M Phillips
Apr 1, 1993·Journal of Virology·D S DimitrovM A Martin
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C BleulC R Mackay
Jul 31, 1998·Micron : the International Research and Review Journal for Microscopy·T GotoK Ikuta
Jul 13, 2000·JAMA : the Journal of the American Medical Association·M P D'SouzaS F Plaeger
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Sep 14, 2000·Current Biology : CB·O T Fackler, B M Peterlin
Jan 24, 2002·AIDS Research and Human Retroviruses·Julià BlancoJosé A Esté
Oct 22, 2002·Journal of Virology·Brenda L FredericksenJ Victor Garcia
Oct 26, 2002·Science·Michael L Dustin, David R Colman
Feb 18, 2003·Science·Tadahiko IgakuraCharles R M Bangham
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
May 6, 2003·Science·David McDonaldThomas J Hope
Aug 28, 2003·Nature Immunology·Etienne Joly, Denis Hudrisier
Jan 22, 2004·The Journal of Experimental Medicine·Clare JollyQuentin J Sattentau

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Citations

Apr 6, 2012·Immunologic Research·Benjamin K Chen
Feb 25, 2011·Molecular Biology of the Cell·Laura García-ExpósitoAgustín Valenzuela-Fernández
Oct 30, 2013·The Journal of Infectious Diseases·Thomas T Murooka, Thorsten R Mempel
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Julià BlancoJosé A Esté
May 17, 2013·Journal of Virology·Richard D SloanMark A Wainberg
Jun 15, 2012·Journal of Virology·Marc PermanyerJosé A Esté
Nov 3, 2006·Journal of Virology·Marion SourisseauOlivier Schwartz
Feb 10, 2011·Journal of Translational Medicine·Claudia CicalaAnthony S Fauci
Oct 15, 2009·Retrovirology·Isabel PuigdomènechJulià Blanco
Dec 28, 2010·Retrovirology·Björn D KuhlMark A Wainberg
Mar 4, 2014·PloS One·María Isabel ClementeMaría Ángeles Muñoz-Fernández
Aug 21, 2008·Biological Chemistry·Claudia Haller, Oliver T Fackler
Oct 26, 2010·Journal of Visualized Experiments : JoVE·Benjamin DaleBenjamin K Chen
Jun 1, 2014·Annals of Biomedical Engineering·Yin-Ting YehSi-Yang Zheng
Mar 24, 2012·Current Opinion in Virology·Quentin J Sattentau
Nov 4, 2010·European Journal of Cell Biology·Claudia HallerOliver T Fackler
Oct 23, 2010·Trends in Microbiology·Marc PermanyerJosé A Esté
Jan 3, 2013·Immunological Reviews·Benjamin M DaleBenjamin K Chen
Nov 9, 2010·Cellular Microbiology·Nuria Izquierdo-UserosJavier Martinez-Picado
Jun 19, 2013·Immunological Reviews·Deanna A KulpaRafick-Pierre Sékaly
May 10, 2006·Journal of Molecular Biology·Boyan GrigorovDelphine Muriaux
Jan 5, 2013·The Journal of General Virology·Isabel OlivaresCecilio López-Galíndez
Jun 1, 2013·Virology·Rebecca A RussellQuentin J Sattentau
Dec 3, 2014·Microbial Pathogenesis·Ashok Chauhan, Mehrab Khandkar
Apr 7, 2010·Journal of Biomedicine & Biotechnology·Anne AucherJulià Blanco
Mar 15, 2015·Trends in Microbiology·Luis M AgostoWalther Mothes

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