High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector

Plant Biotechnology Journal
Guy L RegnardEdward P Rybicki

Abstract

We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf virus (BeYDV-m) and is replicationally released into plant cells from a recombinant Agrobacterium tumefaciens Ti plasmid. pRIC differs from most other geminivirus-based vectors in that the BeYDV replication-associated elements were included in cis rather than from a co-transfected plasmid, while the BeYDV capsid protein (CP) and movement protein (MP) genes were replaced by an antigen encoding transgene expression cassette derived from the non-replicating A. tumefaciens vector, pTRAc. We tested vector efficacy in Nicotiana benthamiana by comparing transient cytoplasmic expression between pRIC and pTRAc constructs encoding either enhanced green fluorescent protein (EGFP) or the subunit vaccine antigens, human papillomavirus subtype 16 (HPV-16) major CP L1 and human immunodeficiency virus subtype C p24 antigen. The pRIC constructs were amplified in planta by up to two orders of magnitude by replication, while 50% mor...Continue Reading

References

Sep 1, 1995·Trends in Biotechnology·H S Mason, C J Arntzen
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E RigdenM A Rezaian
Aug 1, 1997·The Journal of General Virology·L LiuJ Stanley
Apr 1, 1998·Advances in Virus Research·K E Palmer, E P Rybicki
Apr 18, 2002·FEBS Letters·Luke A SelthM Ali Rezaian
May 1, 2002·Journal of Clinical Microbiology·Yevgeniy Y StudentsovRobert D Burk
Dec 20, 2002·Biotechnology and Bioengineering·Tsafrir S MorHugh S Mason
Jun 5, 2003·International Journal for Parasitology·Stephen J Streatfield, John A Howard
Nov 11, 2003·Genetic Vaccines and Therapy·Helen S GarmoryRichard W Titball
Dec 4, 2003·The Journal of General Virology·Kathleen L Hefferon, Ben Dugdale
Mar 9, 2004·Current Opinion in Plant Biology·Rainer FischerRichard M Twyman
Jun 7, 2005·Advanced Drug Delivery Reviews·Helen S GarmoryRichard W Titball
Sep 28, 2005·Biotechnology and Bioengineering·Xiuren Zhang, Hugh Mason
Jan 9, 2007·Plant Biotechnology Journal·Stephen J Streatfield
Mar 10, 2007·Archives of Virology·R P Halley-StottE P Rybicki
Mar 14, 2007·Vaccine·John R Greenland, Norman L Letvin
Sep 14, 2007·BMC Biotechnology·Thomas O KohlEdward P Rybicki
Jun 25, 2008·BMC Biotechnology·Ann MeyersEdward P Rybicki
Nov 6, 2008·Drug Discovery Today·Edward P Rybicki
Mar 25, 2009·Biotechnology and Bioengineering·Zhong HuangHugh Mason
Sep 3, 2009·Expert Review of Vaccines·Edward P Rybicki

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Citations

Dec 14, 2011·Plant Cell Reports·Sergio Rosales-MendozaLeticia Moreno-Fierros
Feb 2, 2012·Antiviral Therapy·Elizabeth D GerschRobert L Garcea
Feb 19, 2013·Expert Review of Vaccines·Nunzia Scotti, Edward P Rybicki
Aug 28, 2014·Expert Review of Vaccines·Marisela HernándezEdda Sciutto
Aug 3, 2010·Expert Review of Vaccines·Colomba GiorgiEdward P Rybicki
Aug 16, 2011·Biotechnology Advances·Edward P RybickiJan-Hendrik Groenewald
Dec 1, 2010·Biotechnology Advances·Olawole O ObembeSiva V Reddy
Jun 16, 2015·Plant Biotechnology Journal·Hadrien Peyret, George P Lomonossoff
Dec 3, 2015·Virology Journal·Francisco F P G PêraInga I Hitzeroth
Mar 26, 2016·Frontiers in Plant Science·Rita B SantosTanja Holland
Aug 9, 2013·Plant Biotechnology Journal·Catherine B PineoEdward P Rybicki
Mar 18, 2010·Plant Biotechnology Journal·Edward P Rybicki
Nov 11, 2015·Human Vaccines & Immunotherapeutics·Edward Rybicki
Apr 30, 2015·Biotechnology Advances·J F BuyelR Fischer
Jun 16, 2015·BioMed Research International·Qiang Chen, Huafang Lai
Apr 7, 2012·Biotechnology Advances·C A MartínezJ Rodríguez Talou
Jul 4, 2012·Biotechnology Advances·Hester C T Lotter-StarkRachel K Chikwamba
Jan 7, 2015·Plant Molecular Biology·Jorge A Salazar-GonzálezSergio Rosales-Mendoza
May 7, 2016·Chemical Society Reviews·Amy M Wen, Nicole F Steinmetz
Jul 31, 2016·Journal of Virological Methods·Richard AtkinsonAnn E Meyers
Mar 28, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Gueven EdgueMarkus Sack
Sep 16, 2017·Viruses·Shunmugiah V RameshHanu R Pappu
Nov 24, 2018·Plant Biotechnology Journal·Md Reyazul IslamInhwan Hwang
Jun 16, 2019·Vaccines·Rosamund Chapman, Edward P Rybicki
Jul 20, 2017·Current Medicinal Chemistry·Lilya Kopertekh, Joachim Schiemann
Sep 6, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Edward P Rybicki
Apr 18, 2015·Expert Review of Vaccines·Sergio Rosales-Mendoza, Dania O Govea-Alonso

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