High levels of glucose induce apoptosis in cardiomyocyte via epigenetic regulation of the insulin-like growth factor receptor

Experimental Cell Research
Xi-Yong YuYangxin Li

Abstract

Diabetic hyperglycemia result in cardiovascular complications, but the mechanisms by which high levels of glucose (HG) cause diabetic cardiomyopathy are not known. We investigate whether HG-induced repression of insulin-like growth factor 1 receptor (IGF-1R) mediated by epigenetic modifications is one potential mechanism. We found that HG resulted in decreased IGF-1 receptor (IGF-1R) mRNA levels, and IGF-1R protein when compared with H9C2 rat cardiomyocyte cells incubated in normal glucose. HG also induced apoptosis of H9C2 cells. The effects of HG on reduced expression of IGF-1R and increased apoptosis were blocked by silencing p53 with small interference RNA but not by non-targeting scrambled siRNA. Moreover, HG negatively regulated IGF-1R promoter activity as determined by ChIP analysis, which was dependent on p53 since siRNA-p53 attenuated the effects of HG on IGF-1R promoter activity. HG also increased the association of p53 with histone deacetylase 1 (HDAC1), and decreased the association of acetylated histone-4 with the IGF-1R promoter. Furthermore, HDAC inhibitor relieved the repression of IGF-1R following HG state. These results suggest that HG-induced repression of IGF-1R is mediated by the association of p53 with the...Continue Reading

References

Oct 1, 1995·The International Journal of Biochemistry & Cell Biology·H WernerD Leroith
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Apr 25, 2000·The Journal of Biological Chemistry·L J JuanC W Wu
Sep 6, 2002·Cardiovascular Toxicology·L Cai, Y J Kang
Nov 27, 2002·Circulation·Peter Libby, Jorge Plutzky
Sep 27, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Irit NahorHaim Werner
Jun 30, 2007·The Biochemical Journal·Mary M KavurmaTrevor D Littlewood
Sep 12, 2007·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·F KuetheH R Figulla
Sep 20, 2008·Biochemical and Biophysical Research Communications·Xi-Yong YuYangxin Li

❮ Previous
Next ❯

Citations

Jun 7, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Andrea Baccarelli, Sanjukta Ghosh
Jun 24, 2011·Diabetes Research and Clinical Practice·Gurinder Bir SinghMadhu Khullar
Jun 27, 2014·BioMed Research International·Daniela BastianelliElena De Falco
Nov 17, 2011·Journal of Molecular and Cellular Cardiology·Andrea L PortburyMonte S Willis
Apr 3, 2014·Oxidative Medicine and Cellular Longevity·Yonggang WangLu Cai
May 7, 2013·International Journal of Cardiology·Xi-Yong YuYangxin Li
Sep 22, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Stefanie KlenkeUlrich H Frey
Jul 18, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Kan Hui YiewNeal L Weintraub
Jun 24, 2018·Cellular and Molecular Life Sciences : CMLS·Veera Ganesh Yerra, Andrew Advani
May 1, 2019·Journal of Lipid and Atherosclerosis·Johannes GollmerHeiko Bugger
Nov 13, 2020·Cellular and Molecular Life Sciences : CMLS·Hongbo MenLu Cai
Mar 1, 2021·Lipids in Health and Disease·Roland Akhigbe, Ayodeji Ajayi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.