PMID: 7914183Aug 1, 1994

High levels of transglutaminase expression in doxorubicin-resistant human breast carcinoma cells

International Journal of Cancer. Journal International Du Cancer
Kapil Mehta


Tissue type II transglutaminase (TGase) is a member of the TGase family that catalyzes Ca(2+)-dependent covalent cross-linking of several amines to the gamma-carboxamide group of protein-bound glutamine residues. The degree of therapeutic efficacy or toxicity of drugs may be related to their ability to serve as a substrate for TGase and their covalent linkage to glutamine residues of regulatory proteins through the catalytic action of this enzyme. Here, doxorubicin (adriamycin)-resistant human breast carcinoma MCF-7ADR cells exhibited 40- to 6C-fold higher TGase activity than control drug-sensitive MCF-7WT cells. The same was observed in vivo: a small proportion of tumor cells became positive for TGase after administration of adriamycin-based chemotherapy to patients with breast carcinoma. Similarly, continuous culture of MCF-7WT cells in the presence of adriamycin led to the appearance of the drug-resistant phenotype that was in turn associated with increased expression of TGase. This increase in TGase was specific for adriamycin resistance. Like most known TGase, MCF-7ADR TGase was completely dependent on the presence of Ca2+ for its catalytic activity. Based on its immunoreactivity, the TGase in MCF-7ADR cells was identified...Continue Reading


Jun 20, 1978·Annals of the New York Academy of Sciences·P J Birckbichler, M K Patterson
Nov 11, 1976·Biochimica Et Biophysica Acta·R L Juliano, V Ling
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charles S GreenbergR H Rice
Feb 1, 1990·The Biochemical Journal·E CocuzziS I Chung
Jan 1, 1989·Annual Review of Biochemistry·J A Endicott, V Ling
Jan 1, 1985·Hybridoma·P J BirckbichlerE Conway
Jan 1, 1985·Pharmacology & Therapeutics·J R Riordan, V Ling
Sep 15, 1987·Biochemical Pharmacology·W T Beck
Nov 17, 1988·Biochimica Et Biophysica Acta·J M BownessT Wong
Jan 1, 1988·Advances in Experimental Medicine and Biology·P J DaviesJ P Stein
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H CowanC E Myers
Oct 22, 1971·Nature·H B Bosmann
Apr 1, 1981·Chemico-biological Interactions·A MungikarB Gothoskar


Nov 15, 1996·International Journal of Cancer. Journal International Du Cancer·P UjházyE Mihich
Feb 7, 2008·Cancer Chemotherapy and Pharmacology·Samar S AzabAshraf B Abdel-Naim
Jun 22, 2007·Amino Acids·P Kotsakis, M Griffin
Jan 30, 2008·Breast Cancer Research and Treatment·Samar S AzabAyman Al-Hendy
Aug 11, 2012·Breast Cancer Research and Treatment·Rabia A GilaniAngela H Brodie
Dec 12, 2012·Breast Cancer Research and Treatment·Kevin D Brown
Jul 11, 2006·Clinical & Experimental Metastasis·Christian F SingerAmbros V Huber
Jul 3, 2010·Journal of Neuro-oncology·Lisa M DyerKevin D Brown
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, Kapil Mehta
May 11, 2002·Breast Cancer Research and Treatment·Jack S K ChenKapil Mehta
Oct 12, 2010·Journal of the National Cancer Institute·A M CalcagnoS V Ambudkar
Dec 25, 2009·Annals of Surgical Oncology·Norikatsu MiyoshiMasaki Mori
Oct 17, 2014·Cell Death & Disease·M ZhuYajun Guo
Sep 19, 2009·Journal of Cancer Research and Clinical Oncology·Kang-Seo ParkKyeong-Man Hong
May 16, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Deborah T LeichtDavid G Beer
Nov 26, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Na WangFukang Xie
May 11, 2010·International Journal of Biological Macromolecules·Byeong-Gu HanByung Il Lee
Sep 23, 2008·Cancer Letters·Dae-Seok KimSoo-Youl Kim
Mar 10, 2016·Clinical & Experimental Metastasis·Jin-Chul HeoHeeyeong Cho
Jul 31, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·A Verma, Kapil Mehta
Jun 3, 2004·Journal of Cellular Physiology·Jack S K ChenKapil Mehta
Apr 30, 2013·Advanced Healthcare Materials·F Philipp SeibDavid L Kaplan
Apr 18, 2006·Journal of Comparative Pathology·Joseph J WakshlagRodney L Page
Sep 17, 2013·Biochimica Et Biophysica Acta·Manaswini SivaramakrishnanGary K Shooter
Jun 26, 2012·The International Journal of Biochemistry & Cell Biology·Kaustubh N BhingeYong-Yu Liu
Feb 8, 2011·European Journal of Pharmacology·Jingxuan WangShi Jin
Sep 18, 2004·Cancer Gene Therapy·Tie-gang LiuRong-guang Shao
Sep 22, 2006·The Journal of Biological Chemistry·Sung-Soo ParkSoo-Youl Kim
Nov 20, 2002·Molecular & Cellular Proteomics : MCP·Gregory C AdamBenjamin F Cravatt
Jul 18, 2018·International Journal of Molecular Medicine·Cuiyun LiGuilong Wang
Sep 5, 2001·The Journal of Nutrition·M A Medina
Dec 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kapil MehtaAysegul A Sahin
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·A VermaKapil Mehta

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