High lncRNA HULC expression is associated with poor prognosis and promotes tumor progression by regulating epithelial-mesenchymal transition in prostate cancer

Archives of Medical Science : AMS
Pengyi ZhengZhijun Li

Abstract

Recently, increasing evidence has shown that long non-coding RNAs (lncRNAs) play critical roles in tumor progression and development. However, the expression pattern and biological function of lncRNA HULC (highly upregulated in liver cancer) in prostate cancer (PCa) remain largely unclear. The expression of lncRNA HULC in 53 paired PCa tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The χ2 test was used to explore the association of lncRNA HULC expression with clinicopathologic features. Kaplan-Meier analysis was used to detect the association between HULC expression and overall survival of PCa patients. Furthermore, the function of HULC in cell growth and metastasis was detected in PCa cells. Our data showed that HULC expression was upregulated in PCa tissues and cell lines compared to adjacent non-tumor tissues and the normal prostate cell line RWPE-1 (p < 0.05). High HULC expression was positively associated with advanced clinicopathologic features and poor overall survival (OS) for PCa patients (p < 0.05). HULC inhibition suppressed PCa cell growth and metastasis both in vitro and in vivo (p < 0.05). Furthermore, HULC knockdown reduced N-cadherin and vimentin expression and...Continue Reading

Citations

Sep 7, 2019·Pathology Oncology Research : POR·Prit Benny MalgulwarMehar Chand Sharma
May 7, 2020·Journal of Cellular Physiology·Soudeh Ghafouri-FardMajid Samsami
Nov 17, 2021·Critical Reviews in Food Science and Nutrition·Mina HomayoonfalBahman Yousefi

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Methods Mentioned

BETA
PCR
transfection
flow cytometry
electrophoresis
xenograft
xenografts

Software Mentioned

SPSS
FlowJo

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